定量研究 20 只正常犬皮肤的真菌组及其主要成分。
Quantifying the mycobiome and its major constituents on the skin of 20 normal dogs.
机构信息
Willows Referral Services (part of Linnaeus Veterinary Ltd), Shirley, Solihull, UK.
Animal Skin and Allergy Clinic, Lynnwood, WA.
出版信息
Am J Vet Res. 2023 Aug 7;84(10):1-5. doi: 10.2460/ajvr.23.04.0071. Print 2023 Sep 1.
OBJECTIVE
To report the density, and constituents, of the mycobiome on the skin surface of normal dogs.
ANIMALS
20 normal dogs were recruited for this study, with informed consent in all cases.
METHODS
Flocked swabs were used to sample the skin surface and to sample the skin surface after superficial scraping with a blunted scapula. Both samples were taken within a brass guide with an internal area of 3.5 cm-2. Next-generation DNA sequencing was used to identify and quantify components of the mycobiome.
RESULTS
The median density of the mycobiome was 1.1 X 105 cm-2 (IQR, 27,561, 409,572). Cladosporium spp and Vishniacozyma victoriae were found on all 20 dogs.
CLINICAL RELEVANCE
Knowledge of the density and the composition of the cutaneous mycobiome will increase our understanding of skin biology and may have relevance to future therapeutic trials.
目的
报告正常犬皮肤表面真菌菌群的密度和组成。
动物
本研究招募了 20 只正常犬，所有犬均获得知情同意。
方法
使用植绒拭子采集皮肤表面样本，并用钝刮板浅层刮取皮肤表面后再次采集样本。两个样本均在一个内部面积为 3.5 cm-2 的黄铜引导器内采集。使用下一代 DNA 测序技术来鉴定和定量真菌菌群的组成成分。
结果
真菌菌群的中位数密度为 1.1 X 105 cm-2（IQR，27,561，409,572）。20 只犬的皮肤上均发现了枝孢属和维多利亚酵母属。
临床相关性
了解皮肤真菌菌群的密度和组成将增加我们对皮肤生物学的认识，并且可能与未来的治疗试验有关。
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