Zhang Huan, Kang Jun, Guo Wuyan, Wang Fujie, Guo Mengjiao, Feng Shanshan, Zhou Wuai, Li Jinnan, Tahir Ayesha T, Wang Shaoshan, Du Xinjun, Zhao Hui, Wang Weihua, Zhu Hong, Zhang Bo
TCM Network Pharmacology Department, Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China.
School of Life Sciences, Tianjin University, Tianjin 300072, China.
Chin Herb Med. 2022 Oct 14;15(3):407-420. doi: 10.1016/j.chmed.2022.08.003. eCollection 2023 Jul.
Identifying novel strategies to prevent particulate matter (PM)-induced lung injury is crucial for the reduction of the morbidity of chronic respiratory diseases. The combined intervention represented by herbal formulae for simultaneously targeting multiple pathological processes can provide a more beneficial effect than the single intervention. The aim of this paper is therefore to design a safe and effective medicinal and edible Chinese herbs (MECHs) formula against PM-induced lung injury.
PM-induced oxidative stress, inflammatory response and apoptosis A549 cell model were used to screen anti-oxidant, anti-inflammatory and anti-apoptotic MECHs, respectively. A network pharmacology method was utilized to rationally design a novel herbal formula. Ultra performance liquid chromatography-mass spectrometer was utilized to assess the quality control of MECHs formula. The excretion of magnetic iron oxide nanospheres of the MECHs formula was estimated in zebrafish. The MECH formula against PM-induced lung injury was investigated with mice experiments.
Five selected herbs were rationally designed to form a new MECH formula, including (Juhong), (Baibiandou), (Baizhu), (Sangye) and (Yuzhu). The formula effectively promoted the magnetic iron oxide nanospheres excretion in zebrafish. The mid/high dose formula significantly prevented PM-induced lung damage in mice by enhancing the activity of SOD and GSH-Px, reducing the MDA and ROS level and attenuating the upregulation of pro-inflammatory cytokine (IL-6, IL-8, IL-1β and TNF-α), down regulating the protein expression of NF-κB, STAT3 and Caspase-3.
Our findings suggest that the effective MECHs formula will become a novel strategy for preventing PM-induced lung injury and provide a paradigm for the development of functional foods using MECHs.
确定预防颗粒物（PM）所致肺损伤的新策略对于降低慢性呼吸道疾病的发病率至关重要。以中药复方为代表的联合干预同时针对多个病理过程，比单一干预能产生更有益的效果。因此，本文旨在设计一种安全有效的药食同源中药（MECHs）配方，以对抗PM所致的肺损伤。
分别采用PM诱导的氧化应激、炎症反应和凋亡A549细胞模型筛选具有抗氧化、抗炎和抗凋亡作用的MECHs。利用网络药理学方法合理设计一种新的中药配方。采用超高效液相色谱 - 质谱联用仪评估MECHs配方的质量控制。在斑马鱼中评估MECHs配方中磁性氧化铁纳米球的排泄情况。通过小鼠实验研究对抗PM所致肺损伤的MECH配方。
合理设计了由5种选定草药组成的新MECH配方，包括（橘红）、（白扁豆）、（白术）、（桑叶）和（玉竹）。该配方有效促进了斑马鱼体内磁性氧化铁纳米球的排泄。中/高剂量配方通过增强超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH - Px）的活性、降低丙二醛（MDA）和活性氧（ROS）水平以及减弱促炎细胞因子（IL - 6、IL - 8、IL - 1β和肿瘤坏死因子 - α）的上调、下调核因子κB（NF - κB）、信号转导和转录激活因子3（STAT3）以及半胱天冬酶 - （Caspase - 3）的蛋白表达，显著预防了小鼠PM诱导的肺损伤。
我们的研究结果表明，有效的MECHs配方将成为预防PM所致肺损伤的新策略，并为利用MECHs开发功能性食品提供范例。
