Chemical constituents from leaves of .

OBJECTIVE

To investigate the chemical constituents from the leaves of and evaluate their inhibition on lipopolysaccharide (LPS)-activated BV-2 microglia cells.

METHODS

The -BuOH extract of the leaves of was isolated by macroporous adsorption resin, silica gel, ODS, column chromatography and semi-preparative HPLC. The structures of the compounds were identified by MS, NMR, ECD, and other spectroscopic methods. In addition, anti-neuroinflammatory effects of isolated compounds were evaluated by measuring the production of nitric oxide (NO) in over-activated BV-2 cells.

RESULTS

Seventeen compounds, including (7,8)-crataegifin A-4----glucopyranoside (), (8,8')-arctigenin (), arctigenin-4'----glucopyranoside (), (-)-syringaresinol (), syringaresinol-4'----glucopyranoside (), (-)-pinoresinol (), pinoresinol-4'----glucopyranoside (), buddlenol D (), (2,3)-dihydroquercetin (), (2,3)-epicatechin (), (2,3)-catechin (), isovitexin (), naringenin-7----glucopyranoside (), chamaejasmin (), neochamaejasmin B (), isoneochamaejasmin A (), and tomentin-5----glucopyranoside () were isolated and identified. Compounds , and significantly inhibited the release of NO in BV-2 microglia activated by LPS, with IC values of 18.34, 29.33 and 26.30 μmol/L, respectively.

CONCLUSION

Compound is a novel compound, and compounds , , , - are isolated from genus for the first time. In addition, the lignans significantly inhibited NO release and the inhibitory activity was decreased after glycosylation.