Zhao Jin, Lu Ran, Jin Chen, Li Siying, Chen Yulin, Huang Qiaorong, Li Xue, Meng Wentong, Wu Hong, Wen Tianfu, Mo Xianming
Department of Liver Surgery, Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Heliyon. 2023 Jul 17;9(7):e18305. doi: 10.1016/j.heliyon.2023.e18305. eCollection 2023 Jul.
The gene expression networks of a single cell can be used to reveal cell type- and condition-specific patterns that account for cell states, cell identity, and its responses to environmental changes. We applied single cell sequencing datasets to define mRNA patterns and visualized potential cellular capacities among hepatocellular cancer cells. The expressing numbers and levels of genes were highly heterogenous among the cancer cells. The cellular characteristics were dependent strongly on the expressing numbers and levels of genes, especially oncogenes and anti-oncogenes, in an individual cancer cell. The transcriptional activations of oncogenes and anti-oncogenes were strongly linked to inherent multiple cellular programs, some of which oppose and contend against other processes, in a cancer cell. The gene expression networks of multiple cellular programs proliferation, differentiation, apoptosis, autophagy, epithelial-mesenchymal transition, ATP production, and neurogenesis coexisted in an individual cancer cell. The findings give rise a hypothesis that a cancer cell expresses balanced combinations of genes and undergoes a given biological process by rapidly transmuting gene expressing networks.
单个细胞的基因表达网络可用于揭示特定细胞类型和条件下的模式，这些模式决定了细胞状态、细胞身份及其对环境变化的反应。我们应用单细胞测序数据集来定义mRNA模式，并可视化肝细胞癌细胞之间的潜在细胞能力。癌细胞中基因的表达数量和水平高度异质。在单个癌细胞中，细胞特征强烈依赖于基因的表达数量和水平，尤其是癌基因和抑癌基因。癌基因和抑癌基因的转录激活与癌细胞中固有的多个细胞程序密切相关，其中一些程序与其他过程相互对立和竞争。多个细胞程序（增殖、分化、凋亡、自噬、上皮-间质转化、ATP产生和神经发生）的基因表达网络共存于单个癌细胞中。这些发现提出了一个假设，即癌细胞通过快速改变基因表达网络来表达基因的平衡组合，并经历特定的生物学过程。
