School of Biosciencess, Mahatma Gandhi University, Kottayam, Kerala, 686560, India.
Department of Biochemistry, Kuriakose Elias College, Mannanam, Kottayam, Kerala, 686561, India.
Appl Biochem Biotechnol. 2024 Apr;196(4):2318-2331. doi: 10.1007/s12010-023-04633-4. Epub 2023 Aug 4.
Poultry products remain as one of the most popular and extensively consumed foods in the world and the introduction of hydrogen sulfide (HS) producing antibiotic resistant bacterial species into it is an emerging challenge. The current study has been designed to analyze the distribution of antibiotic resistance among the HS producing bacteria isolated from the fecal samples of chickens from different poultry farms. Here, twenty bacterial isolates were selected based on their ability to produce HS on XLD agar, and the16S rDNA sequencing was carried out for their molecular identification. The results showed the isolates as belong to Salmonella spp. and Citrobacter spp. and in the antibiotic susceptibility test (AST), three of the Salmonella strains were found to be resistant to antibiotics such as tetracycline, doxycycline, nalidixic acid, and amikacin. Also, fourteen Citrobacter strains showed resistance towards azithromycin, and furthermore, eleven of them were also resistant to streptomycin. Resistance towards tetracycline was observed among five of the Citrobacter strains, and seven were resistant to doxycycline. Further molecular screening by the PCR has showed three of the Salmonella strains along with eight Citrobacter isolates to have tetA gene along with four of the Citrobacter strains to have co-harbored bla gene. The results on biofilm formation have also demonstrated three Salmonella strains along with nine Citrobacter strains to have the ability to form moderate biofilm. The study thus describes the occurrence of HS producing multidrug-resistant bacteria in poultry feces, which might contribute towards the dissemination of antibiotic resistance genes to other microorganisms including human pathogens with likely risk to treat disease conditions.
家禽产品仍然是世界上最受欢迎和广泛消费的食品之一，将产生硫化氢（HS）的抗生素耐药细菌引入其中是一个新出现的挑战。本研究旨在分析从不同家禽养殖场的鸡粪便样本中分离出的产生 HS 的细菌的抗生素耐药性分布。在这里，根据在 XLD 琼脂上产生 HS 的能力选择了二十个细菌分离株，并对其 16S rDNA 进行测序以进行分子鉴定。结果表明，这些分离株属于沙门氏菌和柠檬酸杆菌，在抗生素药敏试验（AST）中，发现三种沙门氏菌菌株对四环素、强力霉素、萘啶酸和阿米卡星等抗生素具有耐药性。此外，十四株柠檬酸杆菌对阿奇霉素表现出耐药性，进一步研究发现其中 11 株对链霉素也具有耐药性。五株柠檬酸杆菌对四环素耐药，七株对强力霉素耐药。通过 PCR 进一步进行分子筛选，发现三种沙门氏菌菌株和八种柠檬酸杆菌分离株具有 tetA 基因，四种柠檬酸杆菌分离株同时具有 bla 基因。生物膜形成的研究结果也表明，三种沙门氏菌菌株和九种柠檬酸杆菌菌株具有形成中度生物膜的能力。因此，该研究描述了家禽粪便中产生 HS 的多药耐药细菌的发生情况，这可能导致抗生素耐药基因传播到其他微生物，包括人类病原体，可能会对疾病治疗产生风险。
