Department of Botany and Plant Sciences, Center for Plant Cell Biology (CEPCEB), Institute of Integrative Genome Biology (IIGB), University of California, Riverside, CA, USA.
Methods Mol Biol. 2023;2686:293-300. doi: 10.1007/978-1-0716-3299-4_13.
The shoot apical meristems (SAMs) are located at the tip of the shoot apex. The SAM harbors stem cells that divide continually to provide cells for developing above-ground organs. Several important developmental events occur in SAMs, such as stem cell maintenance, organ differentiation, and flowering commitment which are under genetic control. The SAM is a collection of specialized cells organized in specific spatial domains. Deciphering the gene regulatory networks, guided by the developmental and environmental signals, in these discrete cell types is essential to decoding the SAM function. Here, I provide updates to the previously published protocols for the protoplasting and subsequent purification through fluorescence-activated cell sorting (FACS) of SAM cell types (Reddy, Fluorescence activated cell sorting of shoot apical meristem cell types. In: Riechmann JL, Wellmer F (eds) Flower development. Methods in molecular biology, vol 1110. Humana, New York, pp 315-321, 2014), which has provided genome-wide gene expression patterns at a single cell-type resolution.
茎尖分生组织（SAM）位于茎尖。SAM 含有干细胞，这些干细胞不断分裂，为地上器官的发育提供细胞。SAM 中发生了几个重要的发育事件，如干细胞维持、器官分化和开花决定，这些过程都受遗传控制。SAM 是由特定空间域中组织的专门细胞的集合。解析由发育和环境信号指导的这些离散细胞类型中的基因调控网络，对于解码 SAM 功能至关重要。在这里，我提供了对之前发表的通过荧光激活细胞分选（FACS）对 SAM 细胞类型进行原生质体化和随后纯化的方案（Reddy，通过荧光激活细胞分选对茎尖分生组织细胞类型进行分选。在：Riechmann JL，Wellmer F（eds）花发育。方法在分子生物学，第 1110 卷。Humana，纽约，第 315-321 页，2014 年）的更新，该方案提供了单细胞分辨率的全基因组基因表达模式。
