ELO Life Systems, Durham, NC, USA.
Instituto de Biología Molecular y Celular de Plantas (IBMCP) (UPV-CSIC), Valencia, Spain.
Methods Mol Biol. 2023;2686:453-494. doi: 10.1007/978-1-0716-3299-4_22.
The advances in genomics and bioinformatics have made possible the study in non-model plants of phenotypes associated to flower development. Floriculture crops are an interesting source of traits associated to flower development such as the transition between zygomorphic and actinomorphic flowers or the production of flowers with double and triple corollas. In this chapter, we summarize the material and methods for the use of floriculture crops to study flower development using genomic tools, from the sequencing and assembly of a reference genome to QTL and RNA-Seq analysis to search candidate genes associated to specific traits.
基因组学和生物信息学的进步使得研究非模式植物与花发育相关的表型成为可能。花卉作物是与花发育相关性状的一个有趣来源,例如从辐射对称花到左右对称花的转变,或者产生具有双瓣和三瓣花冠的花。在本章中,我们总结了使用花卉作物作为研究花发育的基因组工具的材料和方法,从参考基因组的测序和组装到 QTL 和 RNA-Seq 分析,以寻找与特定性状相关的候选基因。