State Key Laboratory of Plant Cell and Chromosome Engineering and National Center of Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Nat Plants. 2019 Feb;5(2):174-183. doi: 10.1038/s41477-018-0349-9. Epub 2019 Jan 28.
Snapdragon (Antirrhinum majus L.), a member of the Plantaginaceae family, is an important model for plant genetics and molecular studies on plant growth and development, transposon biology and self-incompatibility. Here we report a near-complete genome assembly of A. majus cultivar JI7 (A. majus cv.JI7) comprising 510 Megabases (Mb) of genomic sequence and containing 37,714 annotated protein-coding genes. Scaffolds covering 97.12% of the assembled genome were anchored on eight chromosomes. Comparative and evolutionary analyses revealed that a whole-genome duplication event occurred in the Plantaginaceae around 46-49 million years ago (Ma). We also uncovered the genetic architectures associated with complex traits such as flower asymmetry and self-incompatibility, identifying a unique duplication of TCP family genes dated to around 46-49 Ma and reconstructing a near-complete ψS-locus of roughly 2 Mb. The genome sequence obtained in this study not only provides a representative genome sequenced from the Plantaginaceae but also brings the popular plant model system of Antirrhinum into the genomic age.
金鱼草（Antirrhinum majus L.），车前科的一个成员，是植物遗传学和植物生长发育、转座子生物学和自交不亲和性的分子研究的重要模式植物。在这里，我们报告了金鱼草品种 JI7（A. majus cv.JI7）的近完整基因组组装，该基因组包含 510 兆碱基（Mb）的基因组序列，包含 37714 个注释的蛋白质编码基因。覆盖组装基因组 97.12%的支架被锚定在 8 条染色体上。比较和进化分析表明，大约在 46-49 百万年前（Ma），车前科发生了全基因组复制事件。我们还揭示了与花不对称和自交不亲和等复杂性状相关的遗传结构，确定了大约 46-49 Ma 的 TCP 家族基因的独特重复，并重建了大约 2 Mb 的近完整ψS 基因座。本研究获得的基因组序列不仅提供了车前科测序的代表性基因组，而且使金鱼草这一广受欢迎的植物模式系统进入了基因组时代。
