Department of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.
Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2023;2693:25-38. doi: 10.1007/978-1-0716-3342-7_3.
Heat shock proteins (HSP) are rapidly induced after proteotoxic stresses such as heat shock and accumulate at high concentrations in cells. HSP induction involves primarily a family of heat shock transcription factors (HSF) that bind the heat shock elements of the HSP genes and mediate transcription in trans. We discuss methods for the study of HSP binding to HSP promoters and the consequent increases in HSP gene expression in vitro and in vivo.
热休克蛋白（HSP）在热休克等蛋白毒性应激后迅速诱导产生，并在细胞内积累到高浓度。HSP 的诱导主要涉及一组热休克转录因子（HSF），它们与 HSP 基因的热休克元件结合，并在转录中转导。我们讨论了研究 HSP 与 HSP 启动子结合以及随后在体外和体内增加 HSP 基因表达的方法。
