miR-570 靶向 HSP 伴侣网络的转染和热耐受方法分析。

Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Network.

机构信息

Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

JSPS Overseas Research Fellow, Tokyo, Japan.

出版信息

Methods Mol Biol. 2023;2693:73-79. doi: 10.1007/978-1-0716-3342-7_6.

DOI:10.1007/978-1-0716-3342-7_6

PMID:37540427

Abstract

Heat shock proteins (HSPs) are key stress proteins induced in cells exposed to proteotoxic insult and are critical for thermotolerance. The dynamic network of chaperone interactions, known as the chaperome, contributes significantly to the proteotoxic cell response and the malignant phenotype in cancer. We identified a potent microRNA, miR-570 that could bind the 3'untranslated regions of multiple HSP mRNAs and inhibit HSP synthesis. Here, we will introduce the transfection and thermotolerance methods for analysis of miR-570 targeting the HSP chaperone network.

摘要

热休克蛋白（HSPs）是细胞受到蛋白毒性损伤时诱导产生的关键应激蛋白，对于热耐受至关重要。伴侣蛋白相互作用的动态网络，即伴侣组，对蛋白毒性细胞反应和癌症中的恶性表型有重要贡献。我们发现了一种有效的 microRNA，miR-570，它可以结合多个 HSP mRNA 的 3'非翻译区，并抑制 HSP 的合成。在这里，我们将介绍转染和热耐受方法，用于分析 miR-570 靶向 HSP 伴侣网络。