使用天然聚丙烯酰胺凝胶电泳鉴定细胞系中的 Epichaperomes。
Use of Native-PAGE for the Identification of Epichaperomes in Cell Lines.
机构信息
Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
出版信息
Methods Mol Biol. 2023;2693:175-191. doi: 10.1007/978-1-0716-3342-7_14.
Abstract
Epichaperomes are disease-associated pathologic scaffolds, composed of tightly bound chaperones, co-chaperones, and other factors. They mediate anomalous protein-protein interactions inside cells, which aberrantly affects the function of protein networks, and in turn, cellular phenotypes. Epichaperome study necessitates the implementation of methods that retain these protein complexes in their native cellular states for analysis. Here we describe a protocol for detection and composition analysis of epichaperomes in cell homogenates through native polyacrylamide gel electrophoresis.
摘要
Epichaperomes 是与疾病相关的病理性支架，由紧密结合的伴侣蛋白、共伴侣蛋白和其他因素组成。它们介导细胞内异常的蛋白质-蛋白质相互作用，从而异常影响蛋白质网络的功能，并进而影响细胞表型。Epichaperome 的研究需要采用能够在天然细胞状态下保留这些蛋白质复合物的方法进行分析。在这里，我们描述了通过天然聚丙烯酰胺凝胶电泳检测和分析细胞匀浆中 Epichaperomes 的方法。
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