Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China; Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China; College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China.
Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China; Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China; College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China.
Plant Physiol Biochem. 2023 Sep;202:107924. doi: 10.1016/j.plaphy.2023.107924. Epub 2023 Aug 1.
Euonymus japonicus, a common urban street tree, can withstand winter freezing stress in temperate regions. The apoplast is the space outside the plasma membrane, and the changes of metabolites in apoplast may be involved in plant adaptation to adverse environments. To reveal the molecular mechanism underlying the winter freezing stress tolerance in E. japonicus, the changes in physiological and biochemical indexes, apoplast metabolites, and gene expression in the leaves of E. japonicus in early autumn and winter were analyzed. A total of 300 differentially accumulated metabolites were identified in apoplast fluids in E. japonicus, which were mainly related to flavone and flavonol biosynthesis, and galactose metabolism, amino acid synthesis, and unsaturated fatty acid synthesis. Integrated metabolomics and transcriptomics analysis revealed that E. japonicus adjust apoplast metabolites including flavonoids such as quercetin and kaempferol, and oligosaccharides such as raffinose and stachyose, to adapt to winter freezing stress through gene expression regulation. In addition, the regulation of ABA and SA biosynthesis and signal transduction pathways, as well as the activation of the antioxidant enzymes, also played important roles in the adaptation to winter freezing stress in E. japonicus. The present study provided essential data for understanding the molecular mechanism underlying the adaptation to winter freezing stress in E. japonicus.
扶芳藤是一种常见的城市街道树种，能够耐受温带地区冬季的冰冻胁迫。质外体是质膜以外的空间，质外体代谢物的变化可能参与植物对不利环境的适应。为了揭示扶芳藤适应冬季冰冻胁迫的分子机制，本研究分析了扶芳藤初秋和冬季叶片的生理生化指标、质外体代谢物和基因表达的变化。共鉴定出扶芳藤质外体液中有 300 种差异积累代谢物，主要与类黄酮和黄酮醇生物合成以及半乳糖代谢、氨基酸合成和不饱和脂肪酸合成有关。代谢组学和转录组学的综合分析表明，扶芳藤通过基因表达调控来调节质外体代谢物，包括槲皮素和山奈酚等类黄酮和棉子糖和水苏糖等寡糖，以适应冬季冰冻胁迫。此外，ABA 和 SA 生物合成和信号转导途径的调节以及抗氧化酶的激活也在扶芳藤适应冬季冰冻胁迫中发挥了重要作用。本研究为了解扶芳藤适应冬季冰冻胁迫的分子机制提供了重要数据。
