Chen ChunYu, Wang Ping, Yan Yan, Jiao ZeWei, Xie ShuHao, Li Ye, Di Peng
Chongqing Three Gorges Medical College, Chongqing, China.
Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing, China.
Front Plant Sci. 2024 Jul 29;15:1432563. doi: 10.3389/fpls.2024.1432563. eCollection 2024.
is an important medicinal plant, and flavonoids are one of its main secondary metabolites. In this study, the main roots, fibrous roots, stems, leaves and flowers of were analyzed using transcriptomics and widely targeted metabolomics. Through correlation analysis of transcription and metabolism, the flavonoid biosynthesis pathway in was analyzed, and the accumulation of flavonoid metabolites and the expression of related genes were investigated. Metabolomics revealed a total of 209 flavonoid metabolites in , among which flavonoids, flavonols, flavanones and flavanonols significantly accumulated in the flowers and leaves. Transcriptome sequencing revealed that key genes in the flavonoid pathway exhibited increased expression in the flowers and leaves. The expression patterns of key genes involved in flavonoid biosynthesis, including , , , , , , , and , are consistent with their upstream and downstream metabolites, demonstrating a significant positive correlation among them. In addition, the gene is highly expressed in five tissues of , indicating that PjUGT is one of the key factors for the diversity of flavonoid glycosides. The WGCNA results showed that WRKY transcription factors exist widely in the candidate modules, and it was possible that transcription factors are involved in regulating the expression of key genes involved in flavonoid biosynthesis and the biosynthesis of flavonoid metabolites. This study reveals spatial differences in the accumulation patterns of flavonoid metabolites in different tissues and provides important clues for further understanding the regulatory mechanisms of flavonoid metabolism in , thus contributing to the optimization of germplasm resources of and the promotion of genetic diversity analysis.
是一种重要的药用植物，黄酮类化合物是其主要的次生代谢产物之一。在本研究中，对的主根、须根、茎、叶和花进行了转录组学和广泛靶向代谢组学分析。通过转录与代谢的相关性分析，分析了中的黄酮类生物合成途径，并研究了黄酮类代谢产物的积累及相关基因的表达。代谢组学分析表明中共有209种黄酮类代谢产物，其中黄酮、黄酮醇、黄烷酮和黄烷醇在花和叶中显著积累。转录组测序显示黄酮类途径中的关键基因在花和叶中表达增加。参与黄酮类生物合成的关键基因，包括、、、、、、、和的表达模式与其上游和下游代谢产物一致，表明它们之间存在显著的正相关。此外，基因在的五个组织中高表达，表明PjUGT是黄酮糖苷多样性的关键因素之一。加权基因共表达网络分析（WGCNA）结果表明，WRKY转录因子广泛存在于候选模块中，转录因子可能参与调控黄酮类生物合成关键基因的表达以及黄酮类代谢产物的生物合成。本研究揭示了不同组织中黄酮类代谢产物积累模式的空间差异，为进一步了解中的黄酮类代谢调控机制提供了重要线索，从而有助于优化的种质资源并促进其遗传多样性分析。
