Zheng Changliang, Chen Mengting, Chen Yashu, Qu Yinghong, Shi Wenzheng, Shi Liu, Qiao Yu, Li Xin, Guo Xiaojia, Wang Lan, Wu Wenjin
Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Int J Biol Macromol. 2023 Sep 30;249:126121. doi: 10.1016/j.ijbiomac.2023.126121. Epub 2023 Aug 3.
Polysaccharide-based nanoparticles formed by the polyelectrolyte complexation between chitosan (CS) and flaxseed gum (FG) was developed in this work, and it was further used as a carrier for bighead carp peptide (BCP) delivery. The CS molecular weight (M) of 50 kDa and CS/FG mass ratio of 1:2 at pH 3.5 were optimal conditions for the NP preparation, with the minimum particle size (∼155.1 nm) and the maximum BCP encapsulation efficiency (60.3 %). The BCP-loaded CS/FG NPs exhibited the smallest particle size (175.8 nm). Both CS/FG NPs and CS/FG-BCP NPs exhibited roughly uniform spherical shape. FT-IR spectra confirmed the existence of hydrogen bonds and electrostatic interactions in the nanoparticles. The BCP-loaded NPs displayed a higher thermal stability than BCP. Moreover, the release of BCP was controllable and dose-dependent, following a first-order kinetics model. These findings suggested that our CS/FG NPs are a promising carrier for bioactive peptide delivery.
本研究制备了由壳聚糖（CS）与亚麻籽胶（FG）通过聚电解质络合形成的多糖基纳米颗粒，并将其进一步用作大头鲤肽（BCP）递送的载体。制备纳米颗粒的最佳条件为：壳聚糖分子量（M）为50 kDa，pH 3.5时壳聚糖与亚麻籽胶的质量比为1:2，此时粒径最小（约155.1 nm），BCP包封效率最高（60.3%）。负载BCP的CS/FG纳米颗粒粒径最小（175.8 nm）。CS/FG纳米颗粒和CS/FG-BCP纳米颗粒均呈现大致均匀的球形。傅里叶变换红外光谱证实纳米颗粒中存在氢键和静电相互作用。负载BCP的纳米颗粒比BCP具有更高的热稳定性。此外，BCP的释放是可控的且呈剂量依赖性，符合一级动力学模型。这些研究结果表明，我们制备的CS/FG纳米颗粒是一种很有前景的生物活性肽递送载体。
