Systems Biology and Biosystems Control Lab, Instituto de Automática e Informática Industrial, Universitat Politècnica de València, Spain.
Gene Regulation in Cardiovascular Remodeling and Inflammation Group, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain; Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain.
Crit Rev Oncol Hematol. 2023 Oct;190:104088. doi: 10.1016/j.critrevonc.2023.104088. Epub 2023 Aug 2.
Synthetic biology aims to program living bacteria cells with artificial genetic circuits for user-defined functions, transforming them into powerful tools with numerous applications in various fields, including oncology. Cancer treatments have serious side effects on patients due to the systemic action of the drugs involved. To address this, new systems that provide localized antitumoral action while minimizing damage to healthy tissues are required. Bacteria, often considered pathogenic agents, have been used as cancer treatments since the early 20th century. Advances in genetic engineering, synthetic biology, microbiology, and oncology have improved bacterial therapies, making them safer and more effective. Here we propose six modules for a successful synthetic biology-based bacterial cancer therapy, the modules include Payload, Release, Tumor-targeting, Biocontainment, Memory, and Genetic Circuit Stability Module. These will ensure antitumor activity, safety for the environment and patient, prevent bacterial colonization, maintain cell stability, and prevent loss or defunctionalization of the genetic circuit.
合成生物学旨在为具有用户定义功能的人工遗传电路编程活细菌细胞，将其转化为具有许多应用的强大工具，包括肿瘤学。由于涉及的药物的全身作用，癌症治疗对患者有严重的副作用。为了解决这个问题，需要新的系统，这些系统在最小化对健康组织损伤的同时提供局部抗肿瘤作用。细菌自 20 世纪初以来就被用作癌症治疗方法。遗传工程、合成生物学、微生物学和肿瘤学的进步改善了细菌疗法，使其更安全、更有效。在这里，我们提出了基于合成生物学的细菌癌症治疗的六个模块，这些模块包括有效载荷、释放、肿瘤靶向、生物包容、记忆和遗传电路稳定性模块。这些模块将确保抗肿瘤活性、环境和患者的安全性、防止细菌定植、维持细胞稳定性和防止遗传电路的丢失或失能。
