School of Public Health, Chongqing Medical University, Chongqing, China; Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China.
Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing, China.
Environ Toxicol Pharmacol. 2023 Sep;102:104239. doi: 10.1016/j.etap.2023.104239. Epub 2023 Aug 3.
Cetylpyridinium Chloride (CPC) is a common disinfectant with potential mitochondrial toxicity. However, the effects of CPC on female reproduction remains unclear. In the present study, pregnant mice were exposed to environmentally relevant doses of CPC for 3 days, the effects were evaluated in the female offspring. Maternal exposure to CPC caused loss of oocytes in neonatal ovaries. TEM analysis of neonatal ovaries showed CPC caused aberrant mitochondrial morphology including vacuolated and disorganized structure, reduced functional cristae. In addition, CPC decreased mitochondrial membrane potential in neonatal oocytes. Seahorse analysis showed that CPC hampered mitochondrial reserve, manifested as reduced spare respiratory capacity. Furthermore, CPC damaged mitochondrial function and impaired developmental competence of MII oocytes, suggesting a persisting impact into adulthood. In summary, this is the first known demonstration that maternal exposure to CPC caused mitochondrial disorders in neonatal ovaries and had long-term effects on fertility of the female offspring.
氯化十六烷基吡啶（CPC）是一种常见的具有潜在线粒体毒性的消毒剂。然而，CPC 对女性生殖的影响尚不清楚。在本研究中，怀孕小鼠暴露于环境相关剂量的 CPC 3 天，评估其对雌性后代的影响。母体暴露于 CPC 导致新生卵巢中卵母细胞丢失。对新生卵巢的 TEM 分析显示，CPC 导致线粒体形态异常，包括空泡化和结构紊乱，功能嵴减少。此外，CPC 降低了新生卵母细胞中的线粒体膜电位。 Seahorse 分析表明，CPC 阻碍了线粒体储备能力，表现为备用呼吸能力降低。此外，CPC 损害了 MII 卵母细胞的线粒体功能和发育能力，表明其对成年后的影响持续存在。总之，这是首次已知的证明，母体暴露于 CPC 会导致新生卵巢中线粒体紊乱，并对雌性后代的生育能力产生长期影响。
