State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
Angew Chem Int Ed Engl. 2023 Sep 11;62(37):e202218643. doi: 10.1002/anie.202218643. Epub 2023 Aug 4.
In their recent Angewandte Chemie publication (doi: 10.1002/anie.202112063), Cen, Wang, Zhou et al. reported the crystal structure of a ternary complex of the non-heme iron endoperoxidase FtmOx1 (PDB entry 7ETK). The biochemical data assessed in this study were from a retracted study (doi: 10.1038/nature15519) by Zhang, Liu, Zhang et al.; no additional biochemical data were included, yet there was no discussion on the source of the biochemical data in the report by Cen, Wang, Zhou et al. Based on this new crystal structure and subsequent QM/MM-MD calculations, Cen, Wang, Zhou et al. concluded that their work provided evidence supporting the CarC-like mechanistic model for FtmOx1 catalysis. However, the authors did not accurately describe either the CarC-like model or the COX-like model, and they did not address the differences between them. Further, and contrary to their interpretations in the manuscript, the authors' data are consistent with the COX-like model once the details of the CarC-like and COX-like models have been carefully analyzed.
在他们最近发表于《德国应用化学》的论文（doi：10.1002/anie.202112063）中，岑、王、周等人报道了非血红素铁过氧化物酶 FtmOx1 的三元复合物的晶体结构（PDB 条目 7ETK）。本研究评估的生化数据来自张、刘、张等人的一篇已撤回的研究（doi：10.1038/nature15519）；研究中没有包含其他生化数据，但岑、王、周等人的报告中没有讨论生化数据的来源。基于这个新的晶体结构和随后的 QM/MM-MD 计算，岑、王、周等人得出结论，他们的工作为 FtmOx1 催化的 CarC 样机制模型提供了证据。然而，作者并没有准确描述 CarC 样模型或 COX 样模型，也没有讨论它们之间的差异。此外，与他们在稿件中的解释相反，一旦仔细分析了 CarC 样和 COX 样模型的细节，作者的数据就与 COX 样模型一致。
