Tan M H, Nowak N J, Loor R, Ochi H, Sandberg A A, Lopez C, Pickren J W, Berjian R, Douglass H O, Chu T M
Cancer Invest. 1986;4(1):15-23. doi: 10.3109/07357908609039823.
A primary human pancreatic tumor line (BxPC-3) has been established from a biopsy specimen of a histologically confirmed adenocarcinoma of the body of the pancreas. Tumorigenicity was proven by xenograft in athymic nude mice. Upon re-establishment of tumor xenografts in tissue culture, the epithelial tumor cells retained their original morphology. Histopathologically, the tumors grown in nude mice exhibited the original characteristics of the primary adenocarcinoma in the patient, producing traceable mucin and displaying moderately well to poorly differentiated adenocarcinomas with occasional lymphocytic infiltrations at the tumor peripheries. Furthermore, the tumor xenografts differentially expressed carcinoembryonic antigen, human pancreas cancer-associated antigen, and human pancreas-specific antigen. Karyotyping and glucose-6-phosphate dehydrogenase isoenzyme characterization revealed that this tumor line was of human origin and devoid of HeLa cell contamination. The BxPC-3 tumor line has been maintained for more than four years in our laboratory and represents a valuable model for primary human pancreatic cancer.
一种原发性人胰腺肿瘤细胞系（BxPC-3）是从一名经组织学确诊为胰腺体部腺癌的活检标本中建立的。通过在无胸腺裸鼠体内异种移植证明了其致瘤性。在组织培养中重新建立肿瘤异种移植后，上皮肿瘤细胞保持了其原始形态。组织病理学上，在裸鼠体内生长的肿瘤表现出患者原发性腺癌的原始特征，产生可追踪的黏液，并显示出中度至低度分化的腺癌，肿瘤周边偶尔有淋巴细胞浸润。此外，肿瘤异种移植差异表达癌胚抗原、人胰腺癌相关抗原和人胰腺特异性抗原。核型分析和葡萄糖-6-磷酸脱氢酶同工酶特征表明，该肿瘤细胞系源自人类，且未受到HeLa细胞污染。BxPC-3肿瘤细胞系在我们实验室已保存了四年多，是原发性人胰腺癌的一个有价值的模型。
