Kyriazis A P, Kyriazis A A, Scarpelli D G, Fogh J, Rao M S, Lepera R
Am J Pathol. 1982 Feb;106(2):250-60.
Human pancreatic ductal adenocarcinoma line Capan-1 was studied in tissue culture and the nude mouse. In tissue culture, the neoplastic cells grew as large epithelial-like mucin-producing cells. Subcutaneous and intraperitoneal transplantation of neoplastic cells into nude mice resulted in tumor formation characterized by marked invasiveness and distant metastases. Histologically, the tumor appeared as a well-differentiated mucin-producing adenocarcinoma morphologically resembling the tumor of origin. Chromosomal analysis showed a human karyotype with a chromosome number between 51-61. Lactate dehydrogenase and beta 2-microglobulin used as tumor markers were present in both tissue culture and the serum of tumor-bearing mice. The neoplasm, which was characterized by an increased level of cAMP, had lost completely the ability to respond to secretin stimulation. The tumor grown in the nude mouse was resistant to treatment with 5-fluorouracil, behavior identical to that of the original tumor. Diphtheria toxin resulted in complete tumor destruction. Because Capan-1 tumor grown in the nude mouse shows morphologic, biologic, and biochemical characteristics similar to the tumor of origin, it may be an invaluable tool in furthering understanding of the biology of human pancreatic cancer.
对人胰腺导管腺癌Capan-1细胞系进行了组织培养和裸鼠实验研究。在组织培养中，肿瘤细胞以大型上皮样产黏液细胞的形式生长。将肿瘤细胞皮下和腹腔内移植到裸鼠体内可形成肿瘤，其特征为具有明显的侵袭性和远处转移。组织学上，肿瘤表现为高分化产黏液腺癌，形态学上类似于原发肿瘤。染色体分析显示为人核型，染色体数目在51至61之间。作为肿瘤标志物的乳酸脱氢酶和β2-微球蛋白在组织培养物和荷瘤小鼠血清中均有存在。该肿瘤的特征是cAMP水平升高，已完全丧失对促胰液素刺激的反应能力。在裸鼠体内生长的肿瘤对5-氟尿嘧啶治疗具有抗性，其行为与原发肿瘤相同。白喉毒素可导致肿瘤完全破坏。由于在裸鼠体内生长的Capan-1肿瘤表现出与原发肿瘤相似的形态学、生物学和生物化学特征，它可能是进一步了解人类胰腺癌生物学特性的宝贵工具。
