Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, RJ, 21941-617, Brazil.
Medical Biochemistry Institute, National Center for Nuclear Magnetic Resonance, Universidade Federal Do Rio de Janeiro, 21.941-590, Rio de Janeiro, RJ, Brazil.
Curr Microbiol. 2022 Feb 7;79(3):86. doi: 10.1007/s00284-022-02778-9.
The Streptococcus mutans is commonly find in oral environment in both symbiont and dysbiotic conditions, where for the last one it causes the break in homeostatic balance and, in association with other microorganisms' community, results in dental caries process. Additionally, it is important to determine the low molecular weight metabolites profile from Streptococcus mutans to distinguish the endogenous and exogenous compounds from patient subjected to salivary metabolomic studies. Thus, the objective of the present study was to characterize the in vitro metabolomic profile of the maturation of a single-species Streptococcus mutans biofilm using metabolomic approach by H-nuclear magnetic resonance (NMR) spectroscopy. A distinct metabolomic profile was observed after 2 days of biofilm maturation, independently of the presence of enamel substrate. Sucrose, lactate, and fructose were the main metabolites responsible for the distinction. The sucrose was consumed by S. mutans in higher levels in the initial experimental periods than at 6 days of biofilm growth. Lactate and fructose were the main compounds secreted, regardless of the type of growth, but it was also observed production of propionate, iso-butyrate, and pyruvate. Pyruvate metabolism and glycolysis/gluconeogenesis were the main pathways related to biofilm growth. The results contribute to the determination of compounds that are resulted from oral microbial activity and help to guide further metabolomics studies.
变形链球菌通常存在于口腔环境中，无论是共生状态还是失调状态，在后一种状态下，它会打破体内平衡，并与其他微生物群落一起导致龋齿过程。此外，确定变形链球菌的低分子量代谢物谱对于区分唾液代谢组学研究中患者的内源性和外源性化合物非常重要。因此，本研究的目的是使用代谢组学方法（NMR 波谱）表征单一种类变形链球菌生物膜成熟过程中的体外代谢组学特征。在生物膜成熟 2 天后观察到了独特的代谢组学特征，而与釉质底物的存在无关。蔗糖、乳酸盐和果糖是区分的主要代谢物。在初始实验期间，蔗糖被 S. mutans 消耗的水平高于生物膜生长 6 天。乳酸盐和果糖是主要分泌的化合物，无论生长类型如何，但也观察到丙酸、异丁酸和丙酮酸的产生。丙酮酸代谢和糖酵解/糖异生是与生物膜生长相关的主要途径。这些结果有助于确定源于口腔微生物活动的化合物，并有助于指导进一步的代谢组学研究。
