Exotic and Emerging Avian Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, Agricultural Research Service, USDA, 934 College Station Road, Athens, GA, 30605, USA.
U.S. Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, WI, 53711, USA.
Virology. 2023 Sep;586:122-129. doi: 10.1016/j.virol.2023.07.002. Epub 2023 Jul 5.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is believed to have a zoonotic origin with bats suspected as a natural host. In this work, we individually express the ACE2 of seven bat species including, little brown, great roundleaf, Pearson's horseshoe, greater horseshoe, Brazilian free-tailed, Egyptian rousette, and Chinese rufous horseshoe in DF1 cells and determine their ability to support attachment and replication of SARS-CoV-2 viruses. We demonstrate that the ACE2 receptor of all seven species made DF1 cells permissible to SARS-CoV-2. The level of virus replication differed between bat species and variants tested. The Wuhan lineage SARS-CoV-2 virus replicated to higher titers than either variant virus tested. All viruses tested grew to higher titers in cells expressing the human ACE2 gene compared to a bat ACE2. This study provides a practical in vitromethod for further testing of animal species for potential susceptibility to current and emerging SARS-CoV-2 viruses.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）被认为具有动物源，蝙蝠可能是其自然宿主。在这项工作中，我们分别在 DF1 细胞中表达了包括小棕蝠、大圆耳蝠、巴氏蹄蝠、大足鼠耳蝠、巴西游离尾蝠、埃及果蝠和中华菊头蝠在内的七种蝙蝠物种的 ACE2，并确定了它们支持 SARS-CoV-2 病毒附着和复制的能力。我们证明了七种 ACE2 受体均使 DF1 细胞允许 SARS-CoV-2 附着。不同蝙蝠物种和变体的病毒复制水平不同。武汉谱系 SARS-CoV-2 病毒的复制滴度高于测试的两种变体病毒。与蝙蝠 ACE2 相比，所有测试的病毒在表达人 ACE2 基因的细胞中均生长到更高的滴度。本研究提供了一种实用的体外方法，可进一步测试动物物种对当前和新兴 SARS-CoV-2 病毒的潜在易感性。
