State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao Special Administrative Region of China.
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao Special Administrative Region of China; Macau Institute for Applied Research in Medicine and Health, Taipa, Macao Special Administrative Region of China.
J Pharm Biomed Anal. 2023 Oct 25;235:115603. doi: 10.1016/j.jpba.2023.115603. Epub 2023 Jul 25.
Cordyceps sinensis is a precious medicinal food which has been successfully cultivated indoors. It remains to be investigated for a simultaneous comparison on aqueous components of natural and cultivated samples. Herein, an approach of quantitative nuclear magnetic resonance (qNMR) analysis combined with global spectral deconvolution (GSD) was established for simultaneous quantification of 26 aqueous components in C. sinensis. Processed by GSD, the distorted baselines of H NMR spectra were greatly improved, and overlapped signals were also well separated so as to achieve accurate identification and quantitation of components in C. sinensis. Method validation by UHPLC-QTOF-MS and TOF-SIMS analysis revealed that qNMR combined with GSD is a reliable approach for simultaneous quantification of multiple components including characteristic markers of glutamine, GABA and trehalose in authentic and fake C. sinensis. The well-established qNMR approach can be used for quality assessment of natural and cultivated C. sinensis as well as differentiation from fake ones.
蛹虫草是一种珍贵的药食同源真菌，已成功实现室内人工培养。然而，关于天然品与培养品在水提成分方面的同步比较研究仍较少。本研究采用定量核磁共振（qNMR）分析结合全谱解卷积（GSD）方法，建立了一种同时定量分析 26 种蛹虫草水提成分的方法。经过 GSD 处理，H NMR 谱图的扭曲基线得到了极大的改善，重叠信号也得到了很好的分离，从而能够准确地鉴定和定量蛹虫草中的成分。通过超高效液相色谱-四极杆飞行时间质谱（UHPLC-QTOF-MS）和飞行时间二次离子质谱（TOF-SIMS）分析对方法进行验证，结果表明 qNMR 结合 GSD 是一种可靠的方法，可用于同时定量分析包括蛹虫草中谷氨酰胺、GABA 和海藻糖等特征标志物在内的多种成分。该方法可用于评价天然品和培养品的质量，并能区分真假品。
