State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Clinical Metabolomics Center, China Pharmaceutical University, Nanjing 211198, China.
J Proteomics. 2018 Jun 15;181:24-35. doi: 10.1016/j.jprot.2018.03.029. Epub 2018 Mar 30.
Cordyceps sinensis has gained increasing attention due to its nutritional and medicinal properties. Herein, we employed label-free quantitative mass spectrometry to explore the proteome differences between naturally- and artificially-cultivated C. sinensis. A total of 22,829 peptides with confidence ≥95%, corresponding to 2541 protein groups were identified from the caterpillar bodies/stromata of 12 naturally- and artificially-cultivated samples of C. sinensis. Among them, 165 proteins showed significant differences between the samples of natural and artificial cultivation. These proteins were mainly involved in energy production/conversion, amino acid transport/metabolism, and transcription regulation. The proteomic results were confirmed by the identification of 4 significantly changed metabolites, thus, lysine, threonine, serine, and arginine via untargeted metabolomics. The change tendencies of these metabolites were partly in accordance with changes in abundance of the proteins, which was upstream of their synthetic pathways. In addition, the nutritional value in terms of the levels of nucleosides, nucleotides, and adenosine between the artificially- and naturally-cultivated samples was virtually same. These proteomic data will be useful for understanding the medicinal value of C. sinensis and serve as reference for its artificial cultivation.
C. sinensis is a precious and valued medicinal product, the current basic proteome dataset would provide useful information to understand its development/infection processes as well as help to artificially cultivate it. This work would also provide basic proteome profile for further study of C. sinensis.
由于其营养和药用特性，蛹虫草越来越受到关注。在此，我们采用无标记定量质谱法来探索天然和人工栽培蛹虫草之间的蛋白质组差异。从 12 个天然和人工栽培的蛹虫草幼虫体/子实体中鉴定出了具有置信度≥95%的总共 22829 个肽，对应于 2541 个蛋白质组。其中，165 种蛋白质在天然和人工栽培样品之间存在显著差异。这些蛋白质主要涉及能量产生/转化、氨基酸运输/代谢和转录调控。通过非靶向代谢组学鉴定了 4 种差异显著的代谢物赖氨酸、苏氨酸、丝氨酸和精氨酸，从而验证了蛋白质组学结果。这些代谢物的变化趋势与它们合成途径上游的蛋白质丰度变化部分一致。此外，人工和天然栽培样品之间核苷、核苷酸和腺苷的营养价值几乎相同。这些蛋白质组数据将有助于理解蛹虫草的药用价值，并为其人工栽培提供参考。
蛹虫草是一种珍贵且有价值的药用产品，目前的基本蛋白质组数据集将为了解其发育/感染过程提供有用信息，并有助于对其进行人工栽培。这项工作还将为进一步研究蛹虫草提供基本的蛋白质组谱。
