Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan; Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Biosens Bioelectron. 2023 Oct 15;238:115555. doi: 10.1016/j.bios.2023.115555. Epub 2023 Jul 26.
Acetaldehyde, which is an intermediate product of alcohol metabolism, is known to induce symptoms, including alcohol flushing, vomiting, and headaches in humans. Therefore, real-time monitoring of acetaldehyde levels is crucial to mitigating these health issues. However, current methods for detecting low-concentration gases necessitate the use of complex measurement equipment. In this study, we developed a low-cost, low-detection-limit, enzyme-based electrochemical biosensor for acetaldehyde gas detection that does not require sophisticated equipment. The sensor was constructed by screen-printing electrodes onto a porous polyimide film, using grafted MgO-templated carbon (GMgOC) as working electrode material, carbon for the counter electrode, and silver/silver chloride for the reference electrode. Pyrroloquinoline-quinone-dependent aldehyde dehydrogenase was immobilized on the working electrode, and a chamber was attached to the electrode chip and filled with 1-methoxy-5-methylphenazinium methyl sulfate solution. The sensor can be used to measure acetaldehyde gas concentrations from 0.02 to 0.1 ppm, making it suitable for monitoring human skin gas. This low detection limit was achieved by delivering the analyte through the porous polyimide film on which the electrodes were printed and accumulating acetaldehyde in the mesoporous GMgOC of the working electrode. This mechanism suggests that this sensor design can be adapted to develop other low-detection limit gas sensors, such as those for screening skin gas biomarkers.
乙醛是酒精代谢的中间产物，已知会引起人类出现症状，包括脸红、呕吐和头痛。因此，实时监测乙醛水平对于减轻这些健康问题至关重要。然而，目前检测低浓度气体的方法需要使用复杂的测量设备。在这项研究中，我们开发了一种低成本、低检测限、基于酶的电化学生物传感器，用于检测乙醛气体，而无需使用复杂的设备。该传感器通过在多孔聚酰亚胺薄膜上印刷电极来构建，使用接枝 MgO 模板碳 (GMgOC) 作为工作电极材料、碳作为对电极，以及银/氯化银作为参比电极。吡咯并喹啉醌依赖性醛脱氢酶被固定在工作电极上，并在电极芯片上安装一个腔室，其中填充 1-甲氧基-5-甲基吩嗪甲基硫酸盐溶液。该传感器可用于测量 0.02 至 0.1ppm 的乙醛气体浓度，非常适合监测人体皮肤气体。通过将分析物输送到印刷电极的多孔聚酰亚胺薄膜上，并在工作电极的中孔 GMgOC 中积累乙醛，实现了这种低检测限。该机制表明，这种传感器设计可以适应开发其他低检测限气体传感器，例如用于筛选皮肤气体生物标志物的传感器。
