School of Allied Health, Curtin University, Perth, WA, Australia.
Wal-yan Respiratory Centre, Telethon Kids Institute, Perth, WA, Australia.
BMC Pediatr. 2023 Aug 5;23(1):386. doi: 10.1186/s12887-023-04210-y.
Inflammation and oxidative stress play a key role in the development of bronchopulmonary dysplasia (BPD), possibly contributing to persistent respiratory morbidity after preterm birth. We aimed to assess if inflammatory markers were elevated in exhaled breath condensate (EBC) of infants born very prematurely (< 32 weeks gestation) at 12-16 corrected months of age, and if increased levels were associated with BPD diagnosis and respiratory morbidity.
EBC samples and respiratory questionnaires were collected from 15 term-born infants and 33 preterm-born infants, 12 with a neonatal BPD diagnosis. EBC samples were analysed for leukotriene B4 (inflammation) and 8-isoprostane (oxidative stress) concentrations using enzyme-linked immune-assays. Differences between groups were analysed by Kruskal-Wallis Test with post-hoc comparisons, independent samples t-test or Mann-Whitney U test depending on normality of the data.
Leukotriene B4 and 8-isoprostane levels were elevated in exhaled breath condensate of preterm-born infants compared to those born at term (mean difference [95% CI]; 1.52 [0.45, 2.59], p = 0.02; 0.77 [0.52, 1.02], p < 0.001, respectively). Leukotriene B4 and 8-isoprostane levels were independent of BPD diagnosis and respiratory morbidity over the first year of life.
Infants born very prematurely exhibit elevated markers of airway neutrophilic inflammation and oxidative stress beyond the first year of life, regardless of a neonatal diagnosis of chronic lung disease or respiratory morbidity during infancy. These findings may have implications for future lung health.
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炎症和氧化应激在支气管肺发育不良（BPD）的发展中起着关键作用，可能导致早产儿出生后持续存在呼吸系统疾病。我们旨在评估极低出生体重儿（<32 周妊娠）在 12-16 校正月龄时呼出气冷凝物（EBC）中的炎症标志物是否升高，以及升高的水平是否与 BPD 诊断和呼吸疾病相关。
收集了 15 名足月出生婴儿和 33 名早产儿的 EBC 样本和呼吸问卷，其中 12 名新生儿患有 BPD。使用酶联免疫吸附法分析 EBC 样本中的白三烯 B4（炎症）和 8-异前列腺素（氧化应激）浓度。根据数据的正态性，通过 Kruskal-Wallis 检验进行组间差异分析，并进行事后比较、独立样本 t 检验或 Mann-Whitney U 检验。
与足月出生婴儿相比，早产儿呼出的冷凝物中白三烯 B4 和 8-异前列腺素水平升高（平均差异[95%CI]；1.52[0.45, 2.59]，p=0.02；0.77[0.52, 1.02]，p<0.001）。白三烯 B4 和 8-异前列腺素水平与新生儿期慢性肺部疾病或婴儿期呼吸疾病的诊断无关，且与婴儿期后一年的呼吸疾病无关。
无论新生儿是否患有慢性肺部疾病或婴儿期是否有呼吸疾病，极低出生体重儿在生命的第一年之后都表现出气道中性粒细胞炎症和氧化应激标志物的升高。这些发现可能对未来的肺部健康有影响。
无。
