Department of Systems and Computational Biology, School of Life Sciences, University of Hyderabad, Hyderabad, India.
RNA Biol. 2023 Jan;20(1):563-572. doi: 10.1080/15476286.2023.2242649.
Recent reports show that long non-coding RNAs (lncRNAs) have inefficient splicing and fewer alternative splice variants than mRNAs. Here, we have explored the efficiency of lncRNAs and mRNAs in producing various splice variants, given the number of exons in humans and mice. Intriguingly, lncRNAs produce more splice variants per exon, referred to as Transcript Complexity, than mRNAs. Most lncRNA splice variants are the product of the alternative last exon and exon skipping. LncRNAs and mRNAs with higher transcript complexity have shorter intron lengths. Longer exon length and GC/AG at 5'/3' splice sites are associated with higher transcript complexity in lncRNAs. Lastly, our results indicate that inefficient splicing of lncRNAs may facilitate multiple introns splicing and, thus, more spliced products per exon.
最近的报告表明，长非编码 RNA（lncRNA）的剪接效率比 mRNA 低，并且具有较少的可变剪接变体。在这里，我们根据人类和小鼠的外显子数量，探讨了 lncRNA 和 mRNA 产生各种剪接变体的效率。有趣的是，lncRNA 每外显子产生的剪接变体（称为转录复杂性）比 mRNA 多。大多数 lncRNA 剪接变体是可变最后外显子和外显子跳跃的产物。具有更高转录复杂性的 lncRNA 和 mRNA 具有更短的内含子长度。更长的外显子长度和 5'/3'剪接位点的 GC/AG 与 lncRNA 中的更高转录复杂性相关。最后，我们的结果表明，lncRNA 的低效剪接可能促进多个内含子的剪接，从而导致每个外显子产生更多的剪接产物。
