Revzin A, Ceglarek J A, Garner M M
Anal Biochem. 1986 Feb 15;153(1):172-7. doi: 10.1016/0003-2697(86)90077-1.
The properties of nucleic acid-protein complexes in polyacrylamide gels and in solution have been compared. Transcription by RNA polymerase from a lac DNA template within a gel yields products which are the same as those obtained in solution. Likewise the dissociation rates of DNA-protein complexes in a gel are found to be identical to the solution values. Thus complexes existing in bands during gel electrophoresis behave quite normally. There seems to be no "caging" effect, at least not in a typical 5% polyacrylamide gel (30:1, acrylamide: bisacrylamide); the gel matrix does not hinder separation of DNA and protein molecules which have dissociated.
对核酸 - 蛋白质复合物在聚丙烯酰胺凝胶中和溶液中的性质进行了比较。凝胶内来自乳糖操纵子DNA模板的RNA聚合酶转录产生的产物与在溶液中获得的产物相同。同样，发现凝胶中DNA - 蛋白质复合物的解离速率与溶液中的值相同。因此，在凝胶电泳过程中条带中存在的复合物表现正常。似乎不存在“笼蔽”效应，至少在典型的5%聚丙烯酰胺凝胶（丙烯酰胺：双丙烯酰胺为30:1）中不存在；凝胶基质不会阻碍已解离的DNA和蛋白质分子的分离。
