Ryu S, Garges S, Adhya S
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8582-6. doi: 10.1073/pnas.91.18.8582.
The mechanism by which the cAMP receptor protein (CRP) activates transcription has been investigated using the lac promoter of Escherichia coli. For transcription activation, an interaction between DNA-bound CRP and RNA polymerase is not sufficient. CRP must bind to a site in the same DNA and close to the promoter. CRP action requires an intact spacer DNA to provide a rigid support in building a CRP-RNA polymerase protein bridge or to allow a conformational change in the DNA to be transmitted to the lac promoter using the protein bridge as a structural support.
利用大肠杆菌的乳糖启动子，对环磷酸腺苷受体蛋白（CRP）激活转录的机制进行了研究。对于转录激活而言，结合在DNA上的CRP与RNA聚合酶之间的相互作用并不充分。CRP必须结合到同一DNA上靠近启动子的位点。CRP发挥作用需要完整的间隔DNA，以便在构建CRP - RNA聚合酶蛋白桥时提供刚性支撑，或者利用蛋白桥作为结构支撑，使DNA的构象变化传递至乳糖启动子。
