Department of Biology and Biotechnology Lazzaro Spallanzani, University of Pavia, Via Ferrata 9, 27100, Pavia, Italy.
Molecular Enzymology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747, AG Groningen, the Netherlands. Electronic address: https://twitter.com/fraaije1.
Curr Opin Struct Biol. 2023 Oct;82:102669. doi: 10.1016/j.sbi.2023.102669. Epub 2023 Aug 4.
Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide.
祖先序列重建 (ASR) 提供了对蛋白质序列在进化过程中变化的深入了解。更具体地说，它可以说明特定氨基酸变化如何在蛋白质家族内产生不同的表型。在过去几十年中，它已成为揭示控制酶功能的分子共同基础的强大技术。在这里，我们描述了 ASR 揭示一系列不同蛋白质的催化机制和新兴表型的优势，同时也强调了该方法可以提供的生物技术应用。
