Gou Juxiang, Cai Ming, Li Jingjian, Wang Zhiguo, Liu Yuwei, Tu Ruiting, Ran Yichen, Lin Xiaorui, Li Ka, Xu Jiazhuang
West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu 610041, China.
College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2023 Jul;54(4):782-787. doi: 10.12182/20230760205.
To prepare the hydrogel coating on the surfaces of nasogastric tubes and to evaluate its effect on the insertion of nasogastric tubes in a rabbit model.
The polyacrylamide (PAAm) hydrogel coating was prepared by UV-induced free radical polymerization. The morphology of the PAAm coating and its interfacial bonding with the silicone rubber substrates of nasogastric tubes were observed with scanning electron microscope. The composition of the coating was analyzed by Fourier transform infrared (FTIR) spectrometer and X-ray photoelectron spectrometer (XPS). The water absorption power and stability of the coating were measured by the weighing method. Water contact angle meter was used to measure the wettability of the coating and tribometer was used to determine the friction coefficient of the silicone rubber substrates before and after the modification. The cytotoxicity of the coating on L929 murine fibroblast cell line was explored with CCK-8 assay after 24-h coculturing of the L929 cell line with silicone rubber substrates before and after modification. An animal model of nasogastric tube insertion in New Zealand rabbits was used to evaluate the effect of the lubrication coating by assessing the insertion time and nasal damage.
In this study, PAAm hydrogel coating was prepared and constructed on the surface of silicone rubber nasogastric tubes. The coating, with a three-dimensional network structure, showed strong interfacial bonding with silicone rubber substrates. The appearance of amino and carbonyl groups indicated that the PAAm hydrogel coating was grafted on the surfaces of nasogastric tubes. Before the modification, the silicone rubber substrate essentially did not absorb much water, whereas, after the modification, the silicone rubber substrate showed significant improvement of as much as 2.9% in water absorption. After sonication for 90 min, the weight loss rate was only 0.15%. Compared with pristine nasogastric tubes, the water contact angle of the modified nasogastric tubes was reduced from 111.9°±2.2° to 58.9°±1.5° ( =22.59, <0.05). In addition, the friction coefficient of silicone rubber nasogastric tubes decreased by 69.3% from 0.378±0.05 to 0.116±0.004 ( =42.80, <0.05) after modification. Moreover, there was no significant difference in the cytocompatibility between L929 cells cocultured with pristine nasogastric tube and those cocultured with modified nasogastric tube. The animal experiment of nasogastric tube insertion showed that the insertion time of the modified nasogastric tubes was reduced from (41.6±7.8) s to (12.4±2.9) s ( =8.509, <0.05). Laryngoscopy revealed that the PAAm hydrogel coating significantly reduced the mucosal damage caused by the insertion of nasogastric tubes.
In this study, PAAm hydrogel coating with strong interfacial bonding was prepared on the surface of silicone rubber nasogastric tubes. The coating has excellent hydrophilic lubrication property and cytocompatibility, effectively shortens the insertion time, and reduces the damage caused by nasogastric tube insertion.
在鼻胃管表面制备水凝胶涂层，并在兔模型中评估其对鼻胃管插入的影响。
通过紫外线诱导自由基聚合制备聚丙烯酰胺（PAAm）水凝胶涂层。用扫描电子显微镜观察PAAm涂层的形态及其与鼻胃管硅橡胶基质的界面结合情况。用傅里叶变换红外（FTIR）光谱仪和X射线光电子能谱仪（XPS）分析涂层的组成。采用称重法测定涂层的吸水能力和稳定性。用水接触角测量仪测量涂层的润湿性，用摩擦磨损试验机测定改性前后硅橡胶基质的摩擦系数。在L929小鼠成纤维细胞系与改性前后的硅橡胶基质共培养24小时后，用CCK-8法检测涂层对L929小鼠成纤维细胞系的细胞毒性。采用新西兰兔鼻胃管插入动物模型，通过评估插入时间和鼻腔损伤来评价润滑涂层的效果。
本研究在硅橡胶鼻胃管表面制备并构建了PAAm水凝胶涂层。该涂层具有三维网络结构，与硅橡胶基质表现出很强的界面结合。氨基和羰基的出现表明PAAm水凝胶涂层接枝在鼻胃管表面。改性前，硅橡胶基质基本不吸水，而改性后，硅橡胶基质的吸水率显著提高，高达2.9%。超声处理90分钟后，失重率仅为0.15%。与未改性的鼻胃管相比，改性鼻胃管的水接触角从111.9°±2.2°降至58.9°±1.5°（t=22.59，P<0.05）。此外，改性后硅橡胶鼻胃管的摩擦系数从0.378±0.05降至0.116±0.004，降低了69.3%（t=42.80，P<0.05）。此外，与未改性鼻胃管共培养的L929细胞和与改性鼻胃管共培养的L929细胞在细胞相容性方面无显著差异。鼻胃管插入的动物实验表明，改性鼻胃管的插入时间从（41.6±7.8）秒缩短至（12.4±2.9）秒（t=8.509，P<0.05）。喉镜检查显示，PAAm水凝胶涂层显著减少了鼻胃管插入引起的黏膜损伤。
本研究在硅橡胶鼻胃管表面制备了具有强界面结合的PAAm水凝胶涂层。该涂层具有优异的亲水性润滑性能和细胞相容性，有效缩短了插入时间，减少了鼻胃管插入造成的损伤。
