School of Chemical Engineering and Technology, Tianjin University, 300350, Tianjin, China.
Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, 300350, Tianjin, China.
Nat Commun. 2022 Sep 12;13(1):5339. doi: 10.1038/s41467-022-33081-7.
Zwitterionic hydrogels exhibit eminent nonfouling and hemocompatibility. Several key challenges hinder their application as coating materials for blood-contacting biomedical devices, including weak mechanical strength and low adhesion to the substrate. Here, we report a poly(carboxybetaine) microgel reinforced poly(sulfobetaine) (pCBM/pSB) pure zwitterionic hydrogel with excellent mechanical robustness and anti-swelling properties. The pCBM/pSB hydrogel coating was bonded to the PVC substrate via the entanglement network between the pSB and PVC chain. Moreover, the pCBM/pSB hydrogel coating can maintain favorable stability even after 21 d PBS shearing, 0.5 h strong water flushing, 1000 underwater bends, and 100 sandpaper abrasions. Notably, the pCBM/pSB hydrogel coated PVC tubing can not only mitigate the foreign body response but also prevent thrombus formation ex vivo in rats and rabbits blood circulation without anticoagulants. This work provides new insights to guide the design of pure zwitterionic hydrogel coatings for biomedical devices.
两性离子水凝胶表现出显著的抗污和血液相容性。然而，有几个关键挑战阻碍了它们作为与血液接触的生物医学设备的涂层材料的应用，包括弱机械强度和对基底的低附着力。在这里，我们报告了一种具有优异机械强度和抗溶胀性能的聚（羧酸甜菜碱）微凝胶增强聚（磺酸甜菜碱）（pCBM/pSB）两性离子纯水凝胶。pCBM/pSB 水凝胶涂层通过 pSB 和 PVC 链之间的缠结网络与 PVC 基底结合。此外，即使在 PBS 剪切 21 天后、0.5 h 强力水冲洗、1000 次水下弯曲和 100 次砂纸磨损后，pCBM/pSB 水凝胶涂层仍能保持良好的稳定性。值得注意的是，pCBM/pSB 水凝胶涂覆的 PVC 管不仅可以减轻异物反应，而且可以在没有抗凝血剂的情况下防止血栓形成，这在大鼠和兔子的血液循环中得到了证实。这项工作为生物医学设备的纯两性离子水凝胶涂层的设计提供了新的思路。
