Vocational School of Health Services, Giresun University, Giresun, 28200, Türkiye.
Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, 61080, Türkiye.
Chem Biodivers. 2023 Sep;20(9):e202300965. doi: 10.1002/cbdv.202300965. Epub 2023 Aug 23.
In the present study, the volatile composition of Ulva rigida (U. rigida) was elucidated by two different methods. As a result of the identification process of volatile components using the GC/MS-FID instrument, 31 compounds were identified by hydrodistillation (HD) method, and 15 compounds were identified by solid-phase microextraction (SPME) method, elucidating the structure of 99.86 % and 92.65 %, respectively. The most abundant compounds in the essential oil of U. rigida were n-hexadecanoic acid and pentadecanal, while the most abundant compound according to the SPME analysis was heptadecyne, a hydrocarbon compound. In the next step, hexane, dichloromethane, chloroform and methanol solvent extracts of U. rigida were prepared and the antimicrobial activities of the extracts and the essential oil obtained by hydro-distillation as well as the scolicidal activities of the solvent extracts were determined. The results of the antimicrobial activity test of the essential oil showed a high level of activity against Bacillus cereus ATCC 10876 and MRSA. The highest activity was found on the microorganism of Pseudomonas aeruginosa ATCC 9027 in chloroform and methanol extracts of U. rigida. Furthermore, viability detection was performed and the scolicidal effects of the extracts on protoscoleces were assessed. The values of lethal concentration doses (LD , LD and LD ) were calculated using probit analysis.
在本研究中，采用两种不同方法阐明了石莼（U. rigida）的挥发性成分。通过 GC/MS-FID 仪器对挥发性成分进行鉴定，水蒸馏（HD）法鉴定出 31 种化合物，固相微萃取（SPME）法鉴定出 15 种化合物，分别阐明了 99.86%和 92.65%的结构。U. rigida 精油中含量最丰富的化合物是正十六烷酸和十五醛，而根据 SPME 分析，含量最丰富的化合物是十七炔，一种碳氢化合物。下一步，制备了 U. rigida 的正己烷、二氯甲烷、氯仿和甲醇溶剂提取物，并测定了提取物和水蒸馏获得的精油的抗菌活性以及溶剂提取物的杀囊活性。精油的抗菌活性测试结果表明，其对蜡样芽孢杆菌 ATCC 10876 和耐甲氧西林金黄色葡萄球菌（MRSA）具有很高的活性。在 U. rigida 的氯仿和甲醇提取物中，对铜绿假单胞菌 ATCC 9027 这种微生物的活性最高。此外，还进行了生存能力检测，并评估了提取物对原头蚴的杀囊作用。利用概率分析计算致死浓度剂量（LD 50 、LD 90 和 LD 99 ）的值。
