Department Pharmacognosy, Faculty of Pharmacy, Ataturk University, Erzurum 25240, Turkey.
Department Pharmaceutical Botany, Faculty of Pharmacy, Ataturk University, Erzurum 25240, Turkey.
Molecules. 2022 Feb 11;27(4):1228. doi: 10.3390/molecules27041228.
(Hypericaceae) is a genus that comprises a high number of species around the world. In this study, the roots, aerial parts, flowers, fruits, and aerial parts with flowers from were macerated separately by methanol and water and then fractionated by different solvents of, such as ethyl acetate, -hexane, butanol, dichloromethane, aqueous residue sub-extracts, and ethnobotanical use. All the extracts, sub-extracts and essential oils of were investigated for the first time in detail for their antimicrobial, total phenolics, and antioxidant activities. Anatomical structures of the root, stem, leaf, upper and lower leaf surface, stamen, sepal, and petal of were examined. The biochemical layout of essential oils was determined by GC and GC/MS. The antioxidant activity was determined by free radical scavenging activity (by DPPH). Antimicrobial activity was applied against ATCC 10231, ATCC 8739, ATCC 6538, ATCC 19659, and ATCC 750 using microdilution methods. The essentials of the aerial parts, flower, and fruit are characterized by the presence of monoterpene hydrocarbons, whereas roots oil include alkanes. The GC-FID and GC-MS analysis showed that major components of roots, aerial parts, flowers, and fruits oils were undecane (66.1%); α-pinene (17.5%), γ-terpinene (17.4%), and α-thujene (16.9%); α-pinene (55.6%), α-thujene (10.9%), and γ-terpinene (7.7%); α-pinene (85.2%), respectively. The aerial part sub-extracts indicated a greater level of total phenolics and antioxidant potential. The -hexane sub-extracts (from aerial part, flower, and aerial part with flower) showed the best activity against , with 39.06 µg/mL MIC value. The presented research work indicates that can be a novel promising resource of natural antioxidant and antimicrobial compounds.
（玄参科）是一个包含全球大量物种的属。在这项研究中，我们分别用甲醇和水对 的根、地上部分、花、果实和带花的地上部分进行了浸渍，然后用不同的溶剂（如乙酸乙酯、正己烷、正丁醇、二氯甲烷、水残留亚提取物和民族植物学用途）进行了分级分离。我们首次详细研究了 的所有提取物、亚提取物和精油的抗菌、总酚和抗氧化活性。我们还检查了 的根、茎、叶、上下叶面、雄蕊、萼片和花瓣的解剖结构。通过 GC 和 GC/MS 确定了精油的生化结构。抗氧化活性通过自由基清除活性（通过 DPPH）来确定。采用微量稀释法测定对 ATCC 10231、 ATCC 8739、 ATCC 6538、 ATCC 19659 和 ATCC 750 的抗菌活性。地上部分、花和果实的主要成分是单萜烃，而根油则包含烷烃。GC-FID 和 GC-MS 分析表明，根、地上部分、花和果实油的主要成分是十一烷（66.1%）；α-蒎烯（17.5%）、γ-松油烯（17.4%）和α-侧柏烯（16.9%）；α-蒎烯（55.6%）、α-侧柏烯（10.9%）和γ-松油烯（7.7%）；α-蒎烯（85.2%）。地上部分亚提取物显示出更高水平的总酚和抗氧化潜力。正己烷亚提取物（来自地上部分、花和带花的地上部分）对 表现出最佳的活性，MIC 值为 39.06 µg/mL。本研究工作表明， 可能是天然抗氧化和抗菌化合物的新的有前途的资源。
