Faculty of Veterinary Science, Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan.
Faculty of Bioresources and Technology, Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan.
Open Vet J. 2023 Jun;13(6):732-741. doi: 10.5455/OVJ.2023.v13.i6.8. Epub 2023 Jun 10.
Infectious diseases of young and adult birds with respiratory syndrome are a significant deterrent to the development of industrial poultry farming due to decreased productivity and significant mortality. The only effective method of combating viral diseases is timely and targeted vaccination, which largely depends on laboratory diagnostic results.
This article aims to study the real-time reverse transcription polymerase chain reaction, (RT-PCR) which has the prospect of more effective diagnosis of vaccine strains of chicken infectious bronchitis and Newcastle disease.
The fastest and most accurate method for the differential diagnosis of pathogens in an associative viral infection is RT-PCR. The method proposed in the article for selecting primers for amplification made it possible to use this method for the simultaneous interspecies differential diagnosis of two or more viral agents, significantly accelerating their diagnosis.
The correlation of the nucleotide sequence obtained from sequencing to a specific virus strain is complicated by the lack of a single nomenclature mechanism for separating genetic groups.
The results of this study will allow easy and fast typing of sequences into known and databased virus strains and avoid further confusion in the nomenclature of genetic groups in the future.
具有呼吸道综合征的幼鸟和成年鸟类的传染病是工业家禽养殖业发展的重大障碍，因为这会导致生产力下降和死亡率显著上升。对抗病毒性疾病的唯一有效方法是及时和有针对性的疫苗接种，这在很大程度上取决于实验室诊断结果。
本文旨在研究实时逆转录聚合酶链反应（RT-PCR），该方法有望更有效地诊断鸡传染性支气管炎和新城疫的疫苗株。
在关联病毒感染中，用于区分病原体的最快和最准确方法是 RT-PCR。本文提出的用于选择扩增引物的方法使得可以同时对两种或更多种病毒进行种间差异诊断，从而大大加快了诊断速度。
由于缺乏分离遗传群的单一命名机制，测序获得的核苷酸序列与特定病毒株的相关性变得复杂。
本研究的结果将允许将序列轻松快速地分型为已知和基于数据库的病毒株，并避免将来在遗传群的命名法方面进一步混淆。
