Department of Physiology, SDM College of Medical Sciences & Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, India.
Department of Biochemistry/Central Research Lab, K S Hegde Medical Academy, Nitte (Deemed to be University) Mangaluru, India.
Afr Health Sci. 2023 Mar;23(1):309-319. doi: 10.4314/ahs.v23i1.33.
The clinical manifestations of pre-eclampsia are related to placental anti-angiogenic factor alteration. These variations are mainly due to the alteration of plasminolytic components. The study aims to compare the expression of plasminolytic components in the placenta of women with and without pre-eclampsia.
The study included pregnant women with pre-eclampsia as PE group (n = 30) and without pre-eclampsia as a control group (n = 30). Placental bed biopsy tissues were collected. AnxA2, tPA, PAI-1 expression in the placental villous tissue was quantitatively evaluated using immunohistochemistry, western blot, and real time-PCR analysis.
The results of the study showed a significant decrease in the expression of ANXA2 and increased expression of tPA and PAI-1 in PE group compared to control group (p<0.005). AnxA2 expression showed positive correlation with tPA (r=+0.895, p=0.002) and negative correlation with PAI-1(r=-0.905, p=0.020) in control group whereas in the PE group AnxA2 expression was negatively correlated with tPA ((r=-0.801, p=0.016) and PAI-1 (R=-0.831, P=0.010).
Decreased AnxA2 with increased expression of PAI-1 and tPA may be responsible for the altered fibrinolytic activity and play a significant role in pre-eclampsia pathogenesis.
子痫前期的临床表现与胎盘抗血管生成因子改变有关。这些变化主要是由于纤溶成分的改变。本研究旨在比较有和无子痫前期孕妇胎盘的纤溶成分表达。
本研究包括子痫前期孕妇（PE 组，n=30）和无子痫前期孕妇（对照组，n=30）。采集胎盘床活检组织。采用免疫组织化学、Western blot 和实时 PCR 分析定量评估胎盘绒毛组织中 AnxA2、tPA 和 PAI-1 的表达。
研究结果表明，PE 组中 ANXA2 的表达显著降低，tPA 和 PAI-1 的表达增加（p<0.005）。与对照组相比，PE 组中 AnxA2 的表达与 tPA 呈正相关（r=+0.895，p=0.002），与 PAI-1 呈负相关（r=-0.905，p=0.020）；而在对照组中，AnxA2 的表达与 tPA 呈负相关（r=-0.801，p=0.016），与 PAI-1 呈负相关（r=-0.831，p=0.010）。
AnxA2 减少伴 PAI-1 和 tPA 表达增加可能是纤溶活性改变的原因，并在子痫前期发病机制中起重要作用。
