Marvin Tan Xing Haw, Wang Yijie, Zhu Xiongfeng, Mendes Felipe Nanni, Chung Pei-Shan, Chow Yu Ting, Man Tianxing, Lan Hsin, Lin Yen-Ju, Zhang Xiang, Zhang Xiaohe, Nguyen Thang, Ardehali Reza, Teitell Michael A, Deb Arjun, Chiou Pei-Yu
bioRxiv. 2023 Jul 26:2023.07.25.550454. doi: 10.1101/2023.07.25.550454.
We report a large field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13mm at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction stress into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral wave behavior of the cardiac wave.
ONE-SENTENCE SUMMARY: An optical platform for fast, concurrent measurements of cell mechanics at 83 frames per second, over a large area of 13mm .
我们报告了一种大视野高速摄像平台，用于在每秒83帧的速度下测量超过10000个生物细胞在13毫米范围内的亚细胞牵引力。我们的单像素光学示踪剂（SPOT）工具使用嵌入软质底物中的二维衍射光栅，将细胞的机械牵引应力转化为摄像机可检测到的光学颜色。该平台可测量多种细胞类型的亚细胞牵引力，包括紧密连接的组织片和近乎孤立的细胞。我们利用这个平台探索了新生大鼠心室肌细胞（NRVMs）紧密连接片中的机械波传播，发现一些组织区域的激活时间与心脏波的整体螺旋波行为不同。
一个光学平台，可在13毫米的大面积上以每秒83帧的速度快速、同时测量细胞力学。
