Department of Mechanical and Aerospace Engineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States; Department of Bioengineering, University of California Los Angeles, Westwood Plaza, Los Angeles, CA, 90095, United States; Department of Electronics and Photonics, Institute of High Performance Computing (IHPC), Agency for Science, Technology and Research (A*STAR), 1 Fusionopolis Way, 138632, Singapore.
Division of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, 675 Charles E Young Dr S, Los Angeles, CA, 90095, United States.
Biosens Bioelectron. 2024 Aug 15;258:116318. doi: 10.1016/j.bios.2024.116318. Epub 2024 Apr 24.
We report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction force into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral wave behavior of the cardiac wave.
我们报告了一个大规模的视野和高速录像平台，用于测量超过 10000 个生物细胞在 13 毫米范围内以每秒 83 帧的速度产生的亚细胞牵引力。我们的单像素光示踪剂（SPOT）工具使用嵌入软基底的二维衍射光栅将细胞的机械牵引力转换为可通过摄像机检测的光学颜色。该平台可测量多种细胞类型的亚细胞牵引力，包括紧密连接的组织片和接近孤立的细胞。我们使用该平台研究了紧密连接的 Neonatal Rat Ventricular Myocytes（NRVMs）组织片中的机械波传播，发现一些组织区域的激活时间与心脏波的整体螺旋波行为存在不均匀性。
