Tsouris Andreas, Brach Gauthier, Schacherer Joseph, Hou Jing
Université de Strasbourg, CNRS, GMGM UMR 7156, Strasbourg, France.
Institut Universitaire de France (IUF), Paris, France.
bioRxiv. 2023 Jul 25:2023.07.21.550013. doi: 10.1101/2023.07.21.550013.
Gene expression variation, an essential step between genomic variation and phenotypic landscape, is collectively controlled by local () and distant () regulatory changes. Nevertheless, how these regulatory elements differentially influence the heritability of expression traits remains unclear. Here, we bridge this gap by analyzing the transcriptomes of a large diallel panel consisting of 323 unique hybrids originated from genetically divergent yeast isolates. We estimated the broad- and narrow-sense heritability across 5,087 transcript abundance traits and showed that non-additive components account for 36% of the phenotypic variance on average. By comparing allelic expression ratios in the hybrid and the corresponding parental pair, we identified regulatory changes in 25% of all cases, with a majority acting in . We further showed that regulation could underlie coordinated expression variation across highly connected genes, resulting in significantly higher non-additive variance and most likely in some of the missing heritability of gene expression traits.
基因表达变异是基因组变异与表型格局之间的关键步骤，受局部（）和远距离（）调控变化的共同控制。然而，这些调控元件如何差异地影响表达性状的遗传力仍不清楚。在此，我们通过分析由323个源自基因差异较大的酵母分离株的独特杂种组成的大型双列杂交群体的转录组来弥合这一差距。我们估计了5087个转录本丰度性状的广义和狭义遗传力，并表明非加性成分平均占表型变异的36%。通过比较杂种及其相应亲本对中的等位基因表达比率，我们在所有情况的25%中鉴定出调控变化，其中大多数作用于。我们进一步表明，调控可能是高度连接基因间协调表达变异的基础，导致显著更高的非加性变异，并且很可能是基因表达性状一些缺失遗传力的原因。
