Shapiro S H
Stain Technol. 1978 Sep;53(5):269-72. doi: 10.3109/10520297809111944.
Soaking the exposed face of routine paraffin-processed tissue blocks from human autopsies in solutions containing either 60% ethyl alcohol-glycerol 9:1; glycerol-aniline 9:1; 5% aqueous phenol; 5% aqueous Photo-flo, or 10% aqueous ammonium hydroxide for 2--4 hours at 0--4 C resulted in greater ease of ribboning and overall improvement of slide quality in the majority of sections compared to the results with the same blocks soaked only in water at 0--4 C. The use of postembedding adjuvants, especially 10% aqueous ammonium hydroxide, presents a simple, convenient, inexpensive means of increasing section quality with routine as well as refractory paraffin-embedded tissue without sacrificing tissue adhesion, staining qualities or cellular detail.
将人类尸检常规石蜡处理组织块的暴露面浸泡在含有60%乙醇 - 甘油9:1、甘油 - 苯胺9:1、5%苯酚水溶液、5% Photo - flo水溶液或10%氢氧化铵水溶液的溶液中，在0 - 4℃下浸泡2 - 4小时，与仅在0 - 4℃水中浸泡相同组织块的结果相比，大多数切片的条带化更容易，玻片质量总体得到改善。使用包埋后佐剂，特别是10%氢氧化铵水溶液，提供了一种简单、方便、廉价的方法，可提高常规以及难处理的石蜡包埋组织的切片质量，而不会牺牲组织附着力、染色质量或细胞细节。
