Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Obstetrics and Gynecology, Dalian Municipal Women and Children's Medical Group, Dalian, Liaoning, China.
Front Endocrinol (Lausanne). 2023 Jul 18;14:1200002. doi: 10.3389/fendo.2023.1200002. eCollection 2023.
This study aimed to investigate the correlation between vaginal microbiota and pregnancy outcomes of women who achieved pregnancy fertilization (IVF) in Northern China, and to determine a biomarker for evaluation of the risk of preterm births in these women.
In total, 19 women from Northern China women who conceived after IVF and 6 women who conceived naturally were recruited in this study. The vaginal samples of the healthy participants were collected throughout pregnancy, that is, during the first, second, and third trimesters. The V3-V4 region of 16S rRNA was used to analyze the vaginal microbiome, and the bioinformatic analysis was performed using QIIME Alpha and Beta diversity analysis.
Either IVF group or Natural conception group, bacterial community diversities and total species number of vagnal samples from who delivered at term were significantly higher than those who delivered before term. Low abundance of vaginal bacteria indicates an increased risk of preterm delivery. Further, more abundant vaginal bacteria was found in first trimesters instead of the next two trimesters. Vignal samples collected during first trimester showed richer differences and more predictive value for pregnancy outcoes. In addition, the diversity of the vaginal bacterial community decreased as the gestational age increased, in all samples. was only found in participants who conceived after IVF, and the percentage of in viginal samples of normal delivery group is much higher than the samples from preterm delivery group. specifically colonized in vagina of pregnant woman in AFT group (those who conceived after IVF (A), first trimester (F), and delivered at term (T)) and was detected only in women with AFT and AST (those who conceived after IVF (A), second trimester (S), and delivered at term (T)). These data indicates that and have great potential in predicting pregnancy outcomes who pregnanted by vitro fertilization.
Vaginal microbiota were more stable in women who conceived naturally and those who carried pregnancy to term. might act as a positive biomarker, whereas and may act as negative biomarkers for the risk of preterm birth.
本研究旨在探讨中国北方体外受精（IVF）妊娠女性阴道微生物群与妊娠结局的相关性，并确定评估这些女性早产风险的生物标志物。
本研究共纳入 19 名在中国北方通过 IVF 受孕的女性和 6 名自然受孕的女性。健康参与者的阴道样本在整个孕期（即第一、二和三孕期）内采集。使用 16S rRNA 的 V3-V4 区分析阴道微生物组，使用 QIIME Alpha 和 Beta 多样性分析进行生物信息学分析。
无论是 IVF 组还是自然受孕组，足月分娩的阴道样本的细菌群落多样性和总物种数均明显高于早产分娩的阴道样本。阴道细菌丰度低表明早产风险增加。此外，第一孕期的阴道细菌丰度更高，而不是后两个孕期。第一孕期采集的阴道样本显示出更丰富的差异和对妊娠结局的更高预测价值。此外，所有样本中，随着胎龄的增加，阴道细菌群落的多样性逐渐降低。 仅在接受 IVF 受孕的参与者中发现，正常分娩组阴道样本中 的比例明显高于早产分娩组。 具体定植于 AFT 组（接受 IVF 受孕（A）、第一孕期（F）和足月分娩（T）的孕妇阴道），并且仅在 AFT 和 AST 组（接受 IVF 受孕（A）、第二孕期（S）和足月分娩（T）的孕妇阴道）中检测到 。这些数据表明， 和 可能对预测通过体外受精怀孕的妊娠结局具有重要潜力。
自然受孕和足月妊娠的女性阴道微生物群更为稳定。 可能作为正性生物标志物，而 、 可能作为早产风险的负性生物标志物。
