Ghanbarzadeh Nahid, Mohammadparast-Tabas Pouria, Aramjoo Hamed, Allahyari Elahe, Ghasemi Saeedeh, Erfani Soheila, Mesbahzadeh Behzad, Dehghan Hamideh, Zare-Bidaki Majid
Department of Gynecology and Obstetrics, Medical Faculty, Birjand University of Medical Sciences, Birjand, Iran.
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran.
J Reprod Infertil. 2023 Apr-Jun;24(2):101-107. doi: 10.18502/jri.v24i2.12495.
Amniotic fluid in the uterus is beneficial for the fetus growth and protection due to its nutritional elements as well as its antibacterial and anti-inflammatory properties. Today, body membranes are increasingly being used in multiple fields. The purpose of the current study was evaluation of the antibacterial effects of amniotic fluid and comparison of its effects on pathogenic and probiotic bacteria.
This experimental study was conducted on amniotic fluid obtained from 43 healthy mothers who gave birth by selective cesarean section. Then, antibacterial effects of amniotic fluids were investigated on 8 standard bacterial strains, including Bacillus cereus, Escherichia coli, Staphylococcus aureus, Shigella flexneri, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus, and Lactobacillus plantarum by agar well-diffusion method. Data analysis was performed by SPSS software, . 22 (IBM, US).
Amniotic fluid revealed an inhibitory effect on the growth of bacterial strains. Staphylococcus aureus and Streptococcus pyogenes strains showed growth inhibition in 39% and 17% of samples, respectively. In other bacterial strains, there was growth inhibition in less than 5% of the samples. Also, the zone of growth inhibition for Staphylococcus aureus and Streptococcus pyogenes was significantly higher than the other strains. Amniotic fluid samples had an antibacterial effect on all pathogen strains in general, but not on the Lactobacillus plantarum probiotic strain.
Our findings suggest that the antibacterial effect of amniotic fluid on pathogenic bacteria is significantly higher than the Lactobacillus plantarum as a probiotic one. Overall, the findings support the use of natural substances as alternative therapeutic agents to combat antibiotic resistance.
子宫内的羊水因其营养成分以及抗菌和抗炎特性，对胎儿的生长和保护有益。如今，胎膜在多个领域的应用越来越广泛。本研究的目的是评估羊水的抗菌作用，并比较其对病原菌和益生菌的影响。
本实验研究使用的羊水来自43名通过选择性剖宫产分娩的健康母亲。然后，采用琼脂扩散法研究羊水对8种标准菌株的抗菌作用，这些菌株包括蜡样芽孢杆菌、大肠杆菌、金黄色葡萄球菌、福氏志贺菌、铜绿假单胞菌、肺炎克雷伯菌、蜡样芽孢杆菌和植物乳杆菌。数据分析使用SPSS软件（版本22，IBM，美国）进行。
羊水对菌株生长具有抑制作用。金黄色葡萄球菌和化脓性链球菌菌株分别在39%和17%的样本中表现出生长抑制。在其他细菌菌株中，生长抑制出现在不到5%的样本中。此外，金黄色葡萄球菌和化脓性链球菌的生长抑制圈明显高于其他菌株。羊水样本总体上对所有病原菌菌株都有抗菌作用，但对植物乳杆菌益生菌菌株没有作用。
我们的研究结果表明，羊水对病原菌的抗菌作用明显高于作为益生菌的植物乳杆菌。总体而言，这些发现支持使用天然物质作为对抗抗生素耐药性的替代治疗剂。
