Tozetto-Mendoza Tania Regina, da-Costa A Charlys, Moron Antonio F, Leal Élcio, Lima Silvia Helena, Ferreira Noely Evangelista, Honorato Layla, Paião Heuder Gustavo Oliveira, Freire Wilton Santos, Mendes-Correa Maria Cássia, Witkin Steven S
Laboratório de Investigação Médica em Virologia (LIM 52), Faculdade de Medicina da Universidade de São Paulo-Instituto de Medicina Tropical de São Paulo, São Paulo, Brazil.
Department of Obstetrics, Universidade Federal de São Paulo, São Paulo, Brazil.
Front Med (Lausanne). 2023 Jul 20;10:1161091. doi: 10.3389/fmed.2023.1161091. eCollection 2023.
Torquetenovirus (TTV) is a commensal virus present in many healthy individuals. Although considered to be non-pathogenic, its presence and titer have been shown to be indicative of altered immune status in individuals with chronic infections or following allogeneic transplantations. We evaluated if TTV was present in amniotic fluid (AF) at the time of surgery to correct a fetal neurological defect, and whether its detection was predictive of adverse post-surgical parameters. AF was collected from 27 women by needle aspiration prior to a uterine incision. TTV titer in the AF was measured by isolation of viral DNA followed by gene amplification and analysis. The TTV genomes were further characterized and sequenced by metagenomics. Pregnancy outcome parameters were subsequently obtained by chart review. Three of the AFs (11.1%) were positive for TTV at 3.36, 4.16, and 4.19 log copies/mL. Analysis of their genomes revealed DNA sequences similar to previously identified TTV isolates. Mean gestational age at delivery was >2 weeks earlier (32.5 vs. 34.6 weeks) and the prevalence of respiratory distress was greater (100% vs. 20.8%) in the TTV-positive pregnancies. TTV detection in AF prior to intrauterine surgery may indicate elevated post-surgical risk for earlier delivery and newborn respiratory distress.
细小病毒（TTV）是一种存在于许多健康个体中的共生病毒。尽管被认为是非致病性的，但在慢性感染个体或异体移植后，其存在和滴度已被证明可指示免疫状态的改变。我们评估了在进行胎儿神经缺陷矫正手术时羊水中是否存在TTV，以及其检测是否可预测术后不良参数。在子宫切开术前，通过针吸从27名女性中收集羊水。通过分离病毒DNA，随后进行基因扩增和分析来测量羊水中的TTV滴度。通过宏基因组学对TTV基因组进行进一步表征和测序。随后通过查阅病历获得妊娠结局参数。三份羊水样本（11.1%）的TTV呈阳性，滴度分别为3.36、4.16和4.19 log拷贝/毫升。对其基因组的分析揭示了与先前鉴定的TTV分离株相似的DNA序列。在TTV阳性妊娠中，平均分娩孕周早于2周以上（32.5周对34.6周），呼吸窘迫的发生率更高（100%对20.8%）。子宫内手术前羊水中TTV的检测可能表明手术后期早产和新生儿呼吸窘迫的风险升高。
