Tozetto-Mendoza Tania R, Bongiovanni Ann Marie, Minis Evelyn, Linhares Iara M, Boester Allison, Freire Wilton S, Lima Silvia H, de Oliveira Natan P G, Mendes-Correa Maria C, Forney Larry J, Witkin Steven S
Virology laboratory (LIM 52), Tropical Medicine Institute, Medicine School, São Paulo University, Rua Dr. Enéas de Carvalho Aguiar, 470, Cerqueira Cesar, São Paulo, Brazil.
Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, NY, USA.
Reprod Sci. 2020 Nov;27(11):2075-2081. doi: 10.1007/s43032-020-00227-1. Epub 2020 Sep 17.
Vaginal samples from women with term deliveries were tested for torquetenovirus (TTV) by gene amplification, matrix metalloproteinase (MMP)-8 and D- and L-lactic acid by ELISA, and microbiome composition by analysis of the bacterial 16S ribosomal RNA gene. TTV was detected in 43.2%, 31.5%, and 41.4% of first trimester, third trimester, and postpartum samples, respectively. The viral titer was higher in postpartum than in the first (p = 0.0018) or third (p = 0.0013) trimester. The mean gestational age at delivery was lower in women positive for TTV in their first trimester (p = 0.0358). In the first and third trimester, the MMP-8 level was higher if TTV was also present (p < 0.0091). The D-lactic acid level was lower in first trimester samples if TTV was present (p = 0.0334). Lactobacillus crispatus dominance in first and third trimester samples was higher when TTV was absent (p < 0.0033). We conclude that TTV is present in the vagina in many women with normal pregnancy outcomes and that its occurrence is associated with a lack of L. crispatus dominance, an increase in vaginal MMP-8 and a decrease in D-lactic acid.
通过基因扩增检测足月分娩女性的阴道样本中的细小病毒（TTV），采用酶联免疫吸附测定法检测基质金属蛋白酶（MMP）-8以及D-乳酸和L-乳酸，并通过分析细菌16S核糖体RNA基因检测微生物群组成。分别在孕早期、孕晚期和产后样本中检测到TTV的比例为43.2%、31.5%和41.4%。产后的病毒滴度高于孕早期（p = 0.0018）或孕晚期（p = 0.0013）。孕早期TTV呈阳性的女性的平均分娩孕周较低（p = 0.0358）。在孕早期和孕晚期，如果同时存在TTV，MMP-8水平较高（p < 0.0091）。如果存在TTV，孕早期样本中的D-乳酸水平较低（p = 0.0334）。当不存在TTV时，孕早期和孕晚期样本中卷曲乳杆菌的优势度较高（p < 0.0033）。我们得出结论，许多妊娠结局正常的女性阴道中存在TTV，其出现与卷曲乳杆菌优势度缺乏、阴道MMP-8增加和D-乳酸减少有关。
