Le Brian T, Auer Katherine M, Lopez David A, Shum Justin P, Suarsana Brian, Suh Ga-Young Kelly, Hedde Per Niklas, Ahrar Siavash
Department of Biomedical Engineering, California State University Long Beach 1250 Bellflower Blvd. Long Beach, CA 90840, USA.
Beckman Laser Institute and Medical Clinic, University of California Irvine Irvine, CA 92612, USA.
ArXiv. 2023 Jul 24:arXiv:2307.13079v1.
Microscopes are essential for the biomechanical and hydrodynamical investigation of small aquatic organisms. We report a do-it-yourself microscope (GLUBscope) that enables the visualization of organisms from two orthogonal imaging planes - top and side views. Compared to conventional imaging systems, this approach provides a comprehensive visualization strategy of organisms, which could have complex shapes and morphologies. The microscope was constructed by combining custom 3D-printed parts and off-the-shelf components. The system is designed for modularity and reconfigurability. Open-source design files and build instructions are provided in this report. Additionally, proof-of-use experiments (particularly with Hydra) and other organisms that combine the GLUBscope with an analysis pipeline were demonstrated to highlight the system's utility. Beyond the applications demonstrated, the system can be used or modified for various imaging applications.
显微镜对于小型水生生物的生物力学和流体动力学研究至关重要。我们报告了一种自制显微镜（GLUBscope），它能够从两个正交成像平面——顶视图和侧视图对生物体进行可视化。与传统成像系统相比，这种方法提供了一种对形状和形态可能复杂的生物体的全面可视化策略。该显微镜是通过将定制的3D打印部件和现成组件相结合构建而成的。该系统设计用于模块化和可重构性。本报告提供了开源设计文件和构建说明。此外，还展示了使用证明实验（特别是对水螅）以及将GLUBscope与分析管道相结合的其他生物体实验，以突出该系统的实用性。除了已展示的应用外，该系统还可用于或修改用于各种成像应用。
