Le Brian T, Auer Katherine M, Lopez David A, Shum Justin P, Suarsana Brian, Suh Ga-Young Kelly, Hedde Per Niklas, Ahrar Siavash
Department of Biomedical Engineering, California State University Long Beach, 1250 Bellflower Blvd. Long Beach, CA 90840, USA.
Beckman Laser Institute and Medical Clinic, University of California Irvine, Irvine, CA 92612, USA.
HardwareX. 2024 Apr 22;18:e00533. doi: 10.1016/j.ohx.2024.e00533. eCollection 2024 Jun.
Microscopes are essential for the biomechanical and hydrodynamical investigation of small aquatic organisms. We report a prototype of a do-it-yourself microscope that enables the visualization of organisms from two orthogonal imaging planes - top and side views. Compared to conventional imaging systems, this approach provides a comprehensive visualization strategy of organisms, which could have complex shapes and morphologies. The microscope was constructed by combining custom 3D-printed parts and off-the-shelf components. The system is designed for modularity and reconfigurability. Open-source design files and build instructions are provided in this report. Additionally, proof-of-use experiments (particularly with ) and other organisms that combine the imaging with an analysis pipeline were demonstrated to highlight the system's utility. Beyond the applications demonstrated, the system can be used or modified for various imaging applications.
显微镜对于小型水生生物的生物力学和流体动力学研究至关重要。我们报告了一种自制显微镜的原型，它能够从两个正交成像平面——顶视图和侧视图——对生物体进行可视化。与传统成像系统相比，这种方法提供了一种对可能具有复杂形状和形态的生物体的全面可视化策略。该显微镜是通过将定制的3D打印部件和现成组件组合而成的。该系统设计用于模块化和可重构性。本报告提供了开源设计文件和构建说明。此外，还展示了使用证明实验（特别是与……以及其他将成像与分析流程相结合的生物体），以突出该系统的实用性。除了已展示的应用之外，该系统还可用于或修改用于各种成像应用。
