MacGregor A N, Stott D I, Kusel J R
Mol Biochem Parasitol. 1985 Aug;16(2):163-72. doi: 10.1016/0166-6851(85)90084-2.
A number of lectins were assessed for their ability to bind to glycoproteins in the surface membrane of Schistosoma mansoni. The membrane polypeptides were separated by SDS-PAGE and the glycoproteins visualised by incubating the gel with radio-iodinated lectin followed by autoradiography. Most of the individual lectins bound to a variety of glycoproteins but peanut agglutinin and Dolichos biflorus agglutinin bound preferentially to a single glycoprotein of apparent molecular weight 170 000. This glycoprotein was subsequently shown to be exposed at the surface of the parasite and localised at the tubercles.
对多种凝集素结合曼氏血吸虫表面膜糖蛋白的能力进行了评估。通过SDS-PAGE分离膜多肽，并用放射性碘化凝集素孵育凝胶，随后进行放射自显影来观察糖蛋白。大多数单个凝集素能与多种糖蛋白结合，但花生凝集素和双花扁豆凝集素优先结合一种表观分子量为170000的单一糖蛋白。随后证明这种糖蛋白暴露于寄生虫表面并定位于结节处。
