Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
G3 (Bethesda). 2023 Sep 30;13(10). doi: 10.1093/g3journal/jkad179.
Teopod1 (Tp1), Teopod2 (Tp2), and Early phase change (Epc) have profound effects on the timing of vegetative phase change in maize. Gain-of-function mutations in Tp1 and Tp2 delay all known phase-specific vegetative traits, whereas loss-of-function mutations in Epc accelerate vegetative phase change and cause shoot abortion in some genetic backgrounds. Here, we show that Tp1 and Tp2 likely represent cis-acting mutations that cause the overexpression of Zma-miR156j and Zma-miR156h, respectively. Epc is the maize ortholog of HASTY, an Arabidopsis gene that stabilizes miRNAs and promotes their intercellular movement. Consistent with its pleiotropic phenotype and epistatic interaction with Tp1 and Tp2, epc reduces the levels of miR156 and several other miRNAs.
Teopod1 (Tp1)、Teopod2 (Tp2) 和早期相变 (Epc) 对玉米营养生长阶段转变的时间有深远影响。Tp1 和 Tp2 的功能获得性突变延迟了所有已知的特定营养生长性状，而 Epc 的功能丧失性突变加速了营养生长阶段的转变，并在某些遗传背景下导致芽夭折。在这里，我们表明 Tp1 和 Tp2 可能分别代表顺式作用突变，导致 Zma-miR156j 和 Zma-miR156h 的过表达。Epc 是拟南芥基因 HASTY 的玉米同源物，该基因稳定 miRNA 并促进其细胞间运动。与 Epc 的多效表型及其与 Tp1 和 Tp2 的上位性相互作用一致，epc 降低了 miR156 和其他几种 miRNA 的水平。
