Peng Jun, He Yang, He Jun, Zhang Ji-Kun, Yu Zheng-Tao, Xia Ying
Department of neurosurgery, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, Hainan, China.
Department of Neurology, Hainan Medical College First Affiliated Hospital, Haikou, Hainan, China.
Brain Behav. 2023 Oct;13(10):e3204. doi: 10.1002/brb3.3204. Epub 2023 Aug 7.
This study aimed to investigate the treatment effect of G protein-coupled receptor 30 (GPR30) agonist G1 combined with hypothermia (HT) on cognitive impairment and anxiety-like behavior after subarachnoid hemorrhage (SAH) in rats.
Fifty male rats were randomly assigned to one of five groups: Sham group, SAH group, SAH + G1 group, SAH + HT group, and SAH + G1 + HT group. The SAH rat model was established by modified endovascular puncture in all groups except the Sham group. Neurological function after the operation was assessed by Garcia scoring. The degree of rat cerebral edema was determined using dry-wet weighing method on the 28th day after operation. Moreover, the behavioral test was performed on rats on the 4th and 28th days after operation.
Compared with Sham group, the Garcia score of each SAH rat model group decreased significantly on the first day and thereafter increased gradually. However, the recovery rate of each treatment group was higher than the SAH group (no treatment), and the Garcia score of SAH + G1 + HT group was much higher than the SAH group on the seventh day after operation. In addition, each treatment group could obviously reduce the cerebral edema degree of SAH rats, among which rats in SAH + G1 + HT group had lower cerebral edema degree than SAH + G1 group and SAH + HT group. Behavioral test results showed that the combination of GPR30 agonist G1 and HT markedly improved the learning and memory ability of SAH rats, alleviated their anxiety- and emotion-related behavior, and enhanced their social interaction.
GPR30 agonist G1 combined with HT reduces cognitive impairment and anxiety-like behavior in rats with SAH.
本研究旨在探讨G蛋白偶联受体30（GPR30）激动剂G1联合亚低温（HT）对大鼠蛛网膜下腔出血（SAH）后认知障碍和焦虑样行为的治疗效果。
将50只雄性大鼠随机分为五组之一：假手术组、SAH组、SAH + G1组、SAH + HT组和SAH + G1 + HT组。除假手术组外，所有组均通过改良血管内穿刺建立SAH大鼠模型。术后通过Garcia评分评估神经功能。术后第28天采用干湿重法测定大鼠脑水肿程度。此外，在术后第4天和第28天对大鼠进行行为测试。
与假手术组相比，各SAH大鼠模型组的Garcia评分在术后第1天显著降低，此后逐渐升高。然而，各治疗组的恢复率均高于SAH组（未治疗），且SAH + G1 + HT组在术后第7天的Garcia评分远高于SAH组。此外，各治疗组均可明显降低SAH大鼠的脑水肿程度，其中SAH + G1 + HT组大鼠脑水肿程度低于SAH + G1组和SAH + HT组。行为测试结果表明，GPR30激动剂G1与HT联合应用可显著改善SAH大鼠的学习记忆能力，减轻其焦虑和情绪相关行为，并增强其社会交往能力。
GPR30激动剂G1联合HT可减轻SAH大鼠认知障碍和焦虑样行为。
