Ichev K, Ovtscharoff W, Franz H, Mohr J
Histochemistry. 1986;84(2):187-90. doi: 10.1007/BF00499831.
A new two-step method using an Fc-fragment/ferritin conjugate as a marker for the visualization of lectin-binding sites on neuronal and other cell membranes is described. In this study of rat synaptosomes, three lectins were tested: concanavalin A, mistletoe lectin I and wheat germ agglutinin. The specificity of the method was proved by control experiments.
描述了一种新的两步法,该方法使用Fc片段/铁蛋白共轭物作为标记物,以可视化神经元和其他细胞膜上的凝集素结合位点。在这项对大鼠突触体的研究中,测试了三种凝集素:伴刀豆球蛋白A、槲寄生凝集素I和麦胚凝集素。对照实验证明了该方法的特异性。