Key Laboratory of Natural Medicine and Immuno-Engineering of Henan Province, Henan International Joint Laboratory of Medicinal Plants Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, College of Chemistry and Molecular Sciences, Henan University, Zhengzhou 450046, China.
Key Laboratory of Natural Medicine and Immuno-Engineering of Henan Province, Henan International Joint Laboratory of Medicinal Plants Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, College of Chemistry and Molecular Sciences, Henan University, Zhengzhou 450046, China.
Int J Biol Macromol. 2023 Oct 1;250:126155. doi: 10.1016/j.ijbiomac.2023.126155. Epub 2023 Aug 5.
The soluble crude polysaccharides from Dioscorea opposita (DOP1 and DOP2) were prepared and characterized. DOP1 and DOP2 obtained carbohydrate (65.71% and 70.18%, respectively), uronic acid (63.71% and 24.84%, respectively) and protein (8.09% and 9.51%, respectively) with molecular weight of 49.24 kDa and 21.62 kDa, respectively. DOP samples were mainly composed of mannose, glucose, galacturonic acid, galactose, and glucuronic acid. The digestibility in vitro, antioxidant activity and intestinal peristalsis effect were then investigated. DOP1 and DOP2 were degraded with decreased molecular weights (39.58 kDa and 18.56 kDa respectively), increased reducing sugar contents (from 16.95% to 19.27%; 12.45% to 15.50% respectively) and free monosaccharides (from 0.89% to 1.42%; 0.90% to 1.14% respectively) after gastric digestion. Both DOP1 and DOP2 were resistant to intestinal digestion, suggesting that DOP samples can be considered as a dietary fiber. Additionally, DOP1 and DOP2 exhibited antioxidant activities positively correlated with the concentration and remained the activities after gastrointestinal digestion in vitro. Furthermore, DOP reduced the fluorescence intensity significantly, indicating DOP can promote the intestinal peristalsis of zebrafish larvae (5 pdf) at 500 μg/mL. Therefore, DOP1 and DOP2 have a better functionality as dietary fibers, including antioxidant activity and intestinal peristalsis promotion, which can be developed as functional foods.
怀山药（DOP1 和 DOP2）水溶性粗多糖的制备与特性研究。DOP1 和 DOP2 分别获得碳水化合物（65.71%和 70.18%）、糖醛酸（63.71%和 24.84%）和蛋白质（8.09%和 9.51%），分子量分别为 49.24 kDa 和 21.62 kDa。DOP 样品主要由甘露糖、葡萄糖、半乳糖醛酸、半乳糖和葡萄糖醛酸组成。然后研究了它们的体外消化率、抗氧化活性和肠道蠕动作用。DOP1 和 DOP2 在胃消化过程中分子量降低（分别为 39.58 kDa 和 18.56 kDa），还原糖含量增加（分别为 16.95%至 19.27%；12.45%至 15.50%），游离单糖含量增加（分别为 0.89%至 1.42%；0.90%至 1.14%）。DOP1 和 DOP2 均能抵抗肠道消化，提示 DOP 样品可作为膳食纤维。此外，DOP1 和 DOP2 表现出与浓度呈正相关的抗氧化活性，并在体外胃肠消化后保持活性。此外，DOP 显著降低荧光强度，表明 DOP 可在 500 μg/mL 时促进斑马鱼幼鱼的肠道蠕动。因此，DOP1 和 DOP2 作为膳食纤维具有更好的功能特性，包括抗氧化活性和促进肠道蠕动，可以开发为功能性食品。
